LOCUS       sequence-168086-        9974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast and Bacterial shuttle vector, includes origin of transfer for 
            conjugation, used for making a DNA capture vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Wolfgang MC, Kulasekara BR, Liang X, Boyd D, Wu K, Yang Q, Miyada 
            CG, Lory S
  TITLE     Conservation of genome content and virulence determinants among 
            clinical and environmental isolates of Pseudomonas aeruginosa.
  JOURNAL   Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8484-9. Epub 2003 Jun 
            18.
  PUBMED    12815109
REFERENCE   2  (bases 1 to 9974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2003-07-8"; volume: "100(14)"; pages: 
            "8484-9. Epub 2003 Jun 18"
COMMENT     Sequence Label: pLLX13
FEATURES             Location/Qualifiers
     source          1..9974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      11..642
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     complement(2216..2235)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2426..2447
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2710..2732
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2724..2741
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2725..2741
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2782..3285
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     3326..3344
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3382..3404)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3504..3523
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        3547..3761
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             3762..4565
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(4947..4966)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4948..4966)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4985..5001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4985..5001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4998..5020)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5009..5025
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5033..5039,5040..5057,5058..5063))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5078..5099
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             5427..6626
                     /codon_start=1
                     /gene="tetA"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     CDS             complement(7899..9047)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9319..9690)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            9723..9832
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc aggacaactg
      721 gtgagtctgg atctgaagtt cattgttgtc atggatgatc tggaccgact ggagccatcc
      781 caggtggcgg aagtgttcag gcttgtgagt gcagtagccg atctgccccg ctttacccat
      841 attctctgtt atgacaggca gattatcact catgccgttg aacatgcgct gaatatcgaa
      901 gatggcagcc gttatctcca gaaaatcatt cagcttagtt ttaaattacc ccgacctgaa
      961 gcctttgatt tacgtaatga atttcgccag cgggctgagg ctctatatca gcaaattaat
     1021 aatcaaccgc cagactctgg aatggtaagg gatctcatcg cggtgactga tacctatggt
     1081 gccgcacttt cgacgccacg ggaaatccat caggccatta attccctgat ttttctttat
     1141 ccggggatgc gggattttgt ttatttccct gatttgtgcc tgcttcagct tatacgggtg
     1201 acaaacccgg ctctgtatga ctggacagag cattacctga cagaacggtc cgtgattgaa
     1261 accggtcagg gtatgctttc tgacggagag aaagcagact tccgggaggg gcttatcaga
     1321 tgtatgaaga cgttcagggc atcaaatgca gactcgtttc tgacacttgc agactggatc
     1381 tatctcatct gcgcaaggca gaacgtgaag acggccgccc tggacctcgc ccgcgagcgc
     1441 caggcgcacg aggccggcgc gcggacccgc gccacggccc acgagcggac gccgcagcag
     1501 gagcgccaga aggccgccag agaggccgag cgcggccgtg aggcttggac gctagggcag
     1561 ggcatgaaaa agcccgtagc gggctgctac gggcgtctga cgcggtggaa agggggaggg
     1621 gatgttgtct acatggctct gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc
     1681 aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc
     1741 catcgacaat caccgcgagt ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg
     1801 cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct
     1861 cgccggcatc gctgtcgccg gcctgctcct caagcacggc cccaacagtg aagtagctga
     1921 ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa
     1981 gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg
     2041 cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg
     2101 agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt
     2161 cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gcccgctcct
     2221 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     2281 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     2341 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     2401 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     2461 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     2521 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     2581 atttccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     2641 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     2701 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattccat ttccccgaaa
     2761 agtgccacct gggtcctttt catcacgtgc tataaaaata attataattt aaatttttta
     2821 atataaatat ataaattaaa aatagaaagt aaaaaaagaa attaaagaaa aaatagtttt
     2881 tgttttccga agatgtaaaa gactctaggg ggatcgccaa caaatactac cttttatctt
     2941 gctcttcctg ctctcaggta ttaatgccga attgtttcat cttgtctgtg tagaagacca
     3001 cacacgaaaa tcctgtgatt ttacatttta cttatcgtta atcgaatgta tatctattta
     3061 atctgctttt cttgtctaat aaatatatat gtaaagtacg ctttttgttg aaatttttta
     3121 aacctttgtt tatttttttt tcttcattcc gtaactcttc taccttcttt atttactttc
     3181 taaaatccaa atacaaaaca taaaaataaa taaacacaga gtaaattccc aaattattcc
     3241 atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag cgatccgtct aagaaaccat
     3301 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     3361 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     3421 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     3481 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatac
     3541 caccttttca attcatcatt tttttttatt cttttttttg atttcggttt ccttgaaatt
     3601 tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat
     3661 tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc
     3721 aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata
     3781 aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg
     3841 aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt
     3901 tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg
     3961 atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt
     4021 tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg
     4081 cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc
     4141 caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc
     4201 ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa tatactaagg
     4261 gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag
     4321 acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag
     4381 atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg gtctctacag
     4441 gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag
     4501 agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa
     4561 actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa
     4621 tttaattata tcagttatta ccctgcggtg tgaaataccg cacagatgcg taaggagaaa
     4681 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     4741 taaatcagct cattttttaa ccaataggcc gaaatcggca aggatcccta tattaccctg
     4801 ttatccctag cgtaactatc gatctcgaga ttttctagaa cgcgtttaat taacctgtat
     4861 gacccgggct agctctagag catgcgagct cctgcagggt accagatctc tatattaccc
     4921 tgttatccct agcgtaacta agctttccct atagtgagtc gtattagagc ttggcgtaat
     4981 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5041 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5101 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5161 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccggctg ctgaaccccc
     5221 aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt caacaggtcc
     5281 agggcggcac ggatcactgt attcggctgc aactttgtca tgattgacac tttatcactg
     5341 ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt caatcggacc
     5401 agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc gtaattctga
     5461 gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg ccgggcctcc
     5521 tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg ctggcgctgt
     5581 atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat cgtttcgggc
     5641 ggcggccaat cttgctcgtc tcgctggccg gcgccactgt cgactacgcc atcatggcga
     5701 cagcgccttt cctttgggtt ctctatatcg ggcggatcgt ggccggcatc accggggcga
     5761 ctggggcggt agccggcgct tatattgccg atatcactga tggcgatgag cgcgcgcggc
     5821 acttcggctt catgagcgcc tgtttcgggt tcgggatggt cgcgggacct gtgctcggtg
     5881 ggctgatggg cggtttctcc ccccacgctc cgttcttcgc cgcggcagcc ttgaacggcc
     5941 tcaatttcct gacgggctgt ttccttttgc cggagtcgca caaaggcgaa cgccggccgt
     6001 tacgccggga ggctctcaac ccgctcgctt cgttccggtg ggcccggggc atgaccgtcg
     6061 tcgccgccct gatggcggtc ttcttcatca tgcaacttgt cggacaggtg ccggccgcgc
     6121 tttgggtcat tttcggcgag gatcgctttc actgggacgc gaccacgatc ggcatttcgc
     6181 ttgccgcatt tggcattctg cattcactcg cccaggcaat gatcaccggc cctgtagccg
     6241 cccggctcgg cgaaaggcgg gcactcatgc tcggaatgat tgccgacggc acaggctaca
     6301 tcctgcttgc cttcgcgaca cggggatgga tggcgttccc gatcatggtc ctgcttgctt
     6361 cgggtggcat cggaatgccg gcgctgcaag caatgttgtc caggcaggtg gatgaggaac
     6421 gtcaggggca gctgcaaggc tcactggcgg cgctcaccag cctgacctcg atcgtcggac
     6481 ccctcctctt cacggcgatc tatgcggctt ctataacaac gtggaacggg tgggcatgga
     6541 ttgcaggcgc tgccctctac ttgctctgcc tgccggcgct gcgtcgcggg ctttggagcg
     6601 gcgcagggca acgagccgat cgctgatcgt ggaaacgata ggcctatgcc atgcgggtca
     6661 aggcgacttc cggcaagcta tacgcgccct aggagtgcgg ttggaacgtt ggcccagcca
     6721 gatactcccg atcacgagca ggacgccgat gatttgaagc gcactcagcg tctgatccaa
     6781 gaacaaccat cctagcaaca cggcggtccc cgggctgaga aagcccagta aggaaacaac
     6841 tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat
     6901 caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc
     6961 aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa
     7021 ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga
     7081 aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa
     7141 caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat
     7201 cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac
     7261 cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag
     7321 cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg
     7381 tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac
     7441 ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc
     7501 cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc
     7561 gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc
     7621 gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca atcggatctc
     7681 gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt
     7741 caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac
     7801 tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg
     7861 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
     7921 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
     7981 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
     8041 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
     8101 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
     8161 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
     8221 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
     8281 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
     8341 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
     8401 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
     8461 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
     8521 accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
     8581 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
     8641 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc
     8701 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
     8761 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc
     8821 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
     8881 cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg
     8941 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
     9001 tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc
     9061 gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt
     9121 gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg
     9181 ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag ctccgcgaag
     9241 tcgctcttct tgatggagcg catggggacg tgcttggcaa tcacgcgcac cccccggccg
     9301 ttttagcggc taaaaaagtc atggctctgc cctcgggcgg accacgccca tcatgacctt
     9361 gccaagctcg tcctgcttct cttcgatctt cgccagcagg gcgaggatcg tggcatcacc
     9421 gaaccgcgcc gtgcgcgggt cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc
     9481 caggtcgcca ttgatgcggg ccagctcgcg gacgtgctca tagtccacga cgcccgtgat
     9541 tttgtagccc tggccgacgg ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc
     9601 cttttcctca atcgctcttc gttcgtctgg aaggcagtac accttgatag gtgggctgcc
     9661 cttcctggtt ggcttggttt catcagccat ccgcttgccc tcatctgtta cgccggcggt
     9721 agccggccag cctcgcagag caggattccc gttgagcacc gccaggtgcg aataagggac
     9781 agtgaagaag gaacacccgc tcgcgggtgg gcctacttca cctatcctgc ccggctgacg
     9841 ccgttggata caccaaggaa agtctacacg aaccctttgg caaaatcctg tatatcgtgc
     9901 gaaaaaggat ggatataccg aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc
     9961 ggaaaagatc cgtc
//
