LOCUS       sequence-168090-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 5' region of 
            pCALNL_Ch12 reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pHU6_Ch12_62418577-gRNA-rev
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(149..224)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(246..494)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(304..323)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(474..494)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        595..699
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtcat
      121 atgtagagag gtacctcgag aaaaaaaagc accgactcgg tgccactttt tcaagttgat
      181 aacggactag ccttatttta acttgctatt tctagctcta aaacctccct ctcccccaaa
      241 aagtcggtgt ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa
      301 gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca
      361 agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa
      421 ttaattctaa ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg
      481 ggaaataggc cctcttcctg cccgaccttg gatccagtcg aatgaattgg ttcctttaaa
      541 gcctgctttt ttgtacagct agcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      601 acccatattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagggaagc ggtgatcgcc
      721 gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg
      781 ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat
      841 attgatttgc tggttacggt gaccgtaagg ct
//
