LOCUS       sequence-168094-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression vector (with mKate2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pgRNA-CKB
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(100..120)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    124..157
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    410..527
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttccagcata gctcttaaac ggagacgtcc gtctccccaa caaggtggtt ctccaaggga
       61 tacttatagt ctcaaaacac acaattactt tacagttagg gtgagtttcc ttttgtgctg
      121 tttataactt cgtataatgt atgctatacg aagttataga aatccaagcc tatcatgtaa
      181 aatgtagcta gtattaaaaa gaacagatta tctgtctttt atcgcacatt aagcctctat
      241 agttactagg aaatattata tgcaaattaa ccggggcagg ggagtagccg agcttctccc
      301 acaagtccgt gcgagggggc cggcgcgggc ctagagatgg cgcgtcggat ctctagagcg
      361 ggcccggtac taaccaaact ggatctctgc tgtccctgta ataaacccga aaattttgaa
      421 tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt atgtctacta
      481 ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaattg tggatgaata
      541 ctgccatttg tctgcagaat tggcgcacgc agtgccgatc cgttcactaa tcgaatggat
      601 ctgtctctgt ctctctctcc accttcttct tctattcctt cgggcctgtc gggtcccctc
      661 ggggttggga ggtgggtctg aaacgataat ggtgaatatc cctgcctaac tctattcact
      721 atagaaagta cagcaaaaac tattcttaaa cctaccaagc ctcctactat cattatgaat
      781 aattttatat accacagcca atttgttatg ttaaaccaat tccacaaact tgcccattta
      841 tctaattcca ataattcttg ttcatt
//
