LOCUS       sequence-168096-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cloning plasmid derived from loxP reporter. The Kan and pA where 
            digested and removed with XhoI/MluI and replaced with two Esp3I 
            sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Chaikind B, Bessen JL, Thompson DB, Hu JH, Liu DR
  TITLE     A programmable Cas9-serine recombinase fusion protein that operates 
            on DNA sequences in mammalian cells.
  JOURNAL   Nucleic Acids Res. 2016 Aug 11. pii: gkw707.
  PUBMED    27515511
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Aug 11. pii: gkw707."
COMMENT     Sequence Label: pCALNL-eGFP-Esp3I
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     213..234
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      276..285
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(426..445)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(507..529)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
       61 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      121 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      181 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      241 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatcccg
      301 ggcccgcggt accgtcgact gcagaattca atttaaatcg taccgagcga ctcgagcgag
      361 acggatggca gcgtctcaac gcgttcgctc gaattaatca attctttgcc aaaatgatga
      421 gacagcacaa taaccagcac gttgcccagg agctgtagga aaaagaagaa ggcatgaaca
      481 tggttagcag aggctctaga gccgccggtc acacgccaga agccgaaccc cgccctgccc
      541 cgtccccccc gaaggcagcc gtccccccgc ggacagcccc gaggctggag agggagaagg
      601 ggacggcggc gcggcgacgc acgaaggccc tccccgccca tttccttcct gccggcgccg
      661 caccgcttcg ccccgcgccc gctagagggg gtgcggcggc gcctcccaga tttcggctcc
      721 gcacagattt gggacaaag
//
