LOCUS       sequence-168097-         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression vector (with mKate2).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pgRNA-CKB
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(410..463)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atttgagtgg aatgagttct tcaatcgtag ttttgactaa cttgccattc atttctatta
       61 acacaaaaca atctggtgca tagtctgaaa tcaactccct acacatacca caaggactta
      121 ccactcgaat acttctatct acttcgtcag aataagggtg tctaacagct acaatcgtgt
      181 caaaatcctt ttgtccattc gaaactgcac taccaatcgc aatggcttct gcacaaacag
      241 ttactcgtcc tatatacgct tcaatatgta ctgccgaaat gatttctcct gttttcgtac
      301 gaattgccgc tcccacatga tgtttattat cctcataaag cattgtaatc ttctctgtcg
      361 ctacttctac taattctaga tcctgctggg agatgttgaa ggtcttcata gggccgggat
      421 tctcctccac gtcaccgcat gttagaagac ttcctctgcc ctctctgtgc cccagtttgc
      481 tagggaggtc gcagtatctg gccacagcca cctcgtgctg ctcgacgtag gtctctttgt
      541 cggcctcctt gattctttcc agtcttctgt ccacatagta gacgccgggc atcttgaggt
      601 tcttagcggg tttcttggat ctgtatgtgg tcttcaagtt gcagatcagg tggcccccgc
      661 ccacgagctt cagggccatg tcggctctgc cttccaggcc gccgtcagcg gggtacaggg
      721 tctcggtgga ggcctcccag ccgagtgttt tcttctgcat cacagggccg ttggatggga
      781 agttcacccc tctgatcttg acgttgtaga tgaggcagcc gtcctggagg ctggtgtcct
      841 gggtagcggt cagcacgccc ccgtcttcgt atgtggtgac tctctcccat gtgaagccct
      901 cggggaagga ctgcttaaag aagtcg
//
