LOCUS       sequence-168103-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the 
            AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pAAVS1-PDi-CRISPRn
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(55..801)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(833..852)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattcacg cgttttagcc
       61 tggcagcata tcgagatcga agtcatcgag agcgtcagca gggagcatat ccagatcaaa
      121 atcatccagg gcatcggcgg gcagcatgtc caggtcgaag tcgtccaggg cgtcggtggg
      181 tccgccgctc tcgcacttca gctgcttctc gaggccgcag atgatcagtt ccaggccgaa
      241 caggaaggcg ggctcggcgc cctgtctgtc gaacagctcg atggcctgct tcagcagggg
      301 gggcatgctg tcggtggtgg gtgtctctct ttcctctttg gcgacctggt gttcctgttc
      361 ttccagcacg cagcccaggg tgaagtggcc cacggcgctc agggcgtaca gggcgttttc
      421 caggctgaag ccctgctggc acaggaaggc cagctggttt tccagtgtct cgtactgctt
      481 ctcggtgggt ctggtgccca ggtgcacttt ggcgccgtcc cggtggctca gcagggcgca
      541 tctgtagctc ttggcgttgt tccgcaggaa gtcctgccag ctctcgcctt ccagagggca
      601 gctgtgggtg tggtgccggt ccagcatctc gatgggcagg gcgtccagca gggcccgctt
      661 gttcttcacg tgccagtaca gggtgggctg ttccacgccc agtttctggg ccagcttccg
      721 ggtggtcagg ccctcgatgc ccacgccgtt cagcagttcc agggcgctgt tgatcacttt
      781 gctcttgtcc agccggctca tgtcgacatt ctttgccaaa atgatgagac agcacaataa
      841 ccagcacgtt gcccaggagc tgtagga
//
