LOCUS       sequence-168104-         813 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the 
            AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pAAVS1-PDi-CRISPRn
FEATURES             Location/Qualifiers
     source          1..813
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    227..756
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcgaatt cgcccttaga gcagagccag gaacccctgt
      121 agggaagggg caggagagcc aggggcatga gatggtggac gaggaagggg gacagggaag
      181 cctgagcgcc tctcctgggc ttgccaagga ctcaaaccca gaagcccaga gcagggcctt
      241 agggaagcgg gaccctgctc tgggcggagg aatatgtccc agatagcact ggggactctt
      301 taaggaaaga aggatggaga aagagaaagg gagtagaggc ggccacgacc tggtgaacac
      361 ctaggacgca ccattctcac aaagggagtt ttccacacgg acacccccct cctcaccaca
      421 gccctgccag gacggggctg gctactggcc ttatctcaca ggtaaaactg acgcacggag
      481 gaacaatata aattggggac tagaaaggtg aagagccaaa gttagaactc aggaccaact
      541 tattctgatt ttgtttttcc aaactgcttc tcctcttggg aagtgtaagg aagctgcagc
      601 accaggatca gtgaaacgca ccagacagcc gcgtcagagc agctcaggtt ctgggagagg
      661 gtagcgcagg gtggccactg agaaccgggc aggtcacgca tccccccctt ccctcccacc
      721 ccctgccaag ctctccctcc caggatcctc tctggctcca tcgtaagcaa accttagagg
      781 ttctggcaag gagagagatg gctccaggaa atg
//
