LOCUS       sequence-168106-        4103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CNBD1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CNBD1_p.A426S
FEATURES             Location/Qualifiers
     source          1..4103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1503..1602)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1619..1638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1620..1638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1643..1659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1643..1659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2090..2881
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2974..3562
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3463..3482
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3716..3733
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3892..3919)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4011..4097)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgccgatg tcttctcttc cagcagctat tttgtctcac atgacagcta
      241 ttaacaatgt gcctcctcct ccacttcaca gtataccaaa cttgaaaaag tctaagcaca
      301 ttaattatgg ccagttgaat gcattatgcc acattagagg acaacacagc cggagtatga
      361 gcaatatctt atcagctcac gatacattta tgaagcaata tcctaaagta ttcctgcacc
      421 aaaaacccag acttcctaaa cttttcaaac aggaggaaca aagggaactc aatgaaggca
      481 aagaggaaag tcaacatcaa caacctgatg attctaacaa tatagctgtc catgttcaga
      541 gagcacatgg tggccatatt ttatatagac caaaaagagc cacagagaaa tttgaagaat
      601 tcctagctat cttaaagaaa ttgcccattc acaggacgcc atatgaacac aaaactgtgt
      661 ggaagttcct gaaaacaatt ccagatttaa cctttcagct aaatgataag catctgaaaa
      721 cacttagtaa gactgtcttt tccgaaacct ggttgaaagg cagcacagtg gttgcaaatg
      781 atggatttta tgtaatactg aaaggcctag ctcgacctca aacaaacgtg tataaaaatc
      841 tgattgaagg aagtgattca ccagactcgt tcatatctca gagtttccac agcttcattt
      901 ggagtgaaga attcaaaaac tctacacttg ctgagatgta cctaccttca tatgactcaa
      961 tgcttagcaa atggagtacc tttgggactc tggaagttat gcctcagaat gaatcggaaa
     1021 cacagatgtt ctcggtggtg acagaagacg attgtgaaat tcttaaaatc ccagcaaagg
     1081 gatatgcaaa gataaaggag gaaaaaataa aacttgaaaa tatgcaaaag ttgaaattaa
     1141 tccgtatgtg tccttattat gaggaatggc ctactttatc catatatgag ctaattgcac
     1201 tccttaaatg gaaaaaattt cctccaggtc atgtgatagt ggaaagtgga aatataattt
     1261 cttttgtggg ttatattaac tctggatgct gtaacattta tagaagtatt ataggatttg
     1321 tgaaactacg atcaaataaa gtgaaaagat ctcaaaaact tgtttatatg gggaaactta
     1381 aggagaagga gtcctttggt gagattagcg tccttcttca agttcctttc acgtgcacaa
     1441 tcattaccaa aaaagaagtt gagatgtcaa tcattgaaga taaggaccta tttgtggctg
     1501 attgccaact ttcttgtaca aagttgtcat tataagaaag cattgcttat caatttgttg
     1561 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     1621 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     1681 aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc tctagaccag
     1741 ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac gcacaccgtg
     1801 gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     1861 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     1921 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     1981 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     2041 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta tgagggaagc
     2101 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     2161 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     2221 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2281 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2341 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2401 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2461 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2521 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2581 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2641 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2701 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2761 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2821 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     2881 accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc
     2941 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3001 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     3061 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     3121 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     3181 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     3241 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     3301 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     3361 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     3421 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     3481 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3541 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3601 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3661 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3721 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     3781 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     3841 caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca
     3901 tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc
     3961 cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta
     4021 ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga
     4081 gcctttcgtt ttatttgatg cct
//
