LOCUS       sequence-168108-        4104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CNBD1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CNBD1_p.S372N
FEATURES             Location/Qualifiers
     source          1..4104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1504..1603)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1620..1639)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1621..1639)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1644..1660)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1644..1660)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2091..2882
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2975..3563
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3464..3483
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3717..3734
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3893..3920)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4012..4098)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgccgatg tcttctcttc cagcagctat tttgtctcac atgacagcta
      241 ttaacaatgt gcctcctcct ccacttcaca gtataccaaa cttgaaaaag tctaagcaca
      301 ttaattatgg ccagttgaat gcattatgcc acattagagg acaacacagc cggagtatga
      361 gcaatatctt atcagctcac gatacattta tgaagcaata tcctaaagta ttcctgcacc
      421 aaaaacccag acttcctaaa cttttcaaac aggaggaaca aagggaactc aatgaaggca
      481 aagaggaaag tcaacatcaa caacctgatg attctaacaa tatagctgtc catgttcaga
      541 gagcacatgg tggccatatt ttatatagac caaaaagagc cacagagaaa tttgaagaat
      601 tcctagctat cttaaagaaa ttgcccattc acaggacgcc atatgaacac aaaactgtgt
      661 ggaagttcct gaaaacaatt ccagatttaa cctttcagct aaatgataag catctgaaaa
      721 cacttagtaa gactgtcttt tccgaaacct ggttgaaagg cagcacagtg gttgcaaatg
      781 atggatttta tgtaatactg aaaggcctag ctcgacctca aacaaacgtg tataaaaatc
      841 tgattgaagg aagtgattca ccagactcgt tcatatctca gagtttccac agcttcattt
      901 ggagtgaaga attcaaaaac tctacacttg ctgagatgta cctaccttca tatgactcaa
      961 tgcttagcaa atggagtacc tttgggactc tggaagttat gcctcagaat gaatcggaaa
     1021 cacagatgtt ctcggtggtg acagaagacg attgtgaaat tcttaaaatc ccagcaaagg
     1081 gatatgcaaa gataaaggag gaaaaaataa aacttgaaaa tatgcaaaag ttgaaattaa
     1141 tccgtatgtg tccttattat gaggaatggc ctactttatc catatatgag ctaattgcac
     1201 tccttaaatg gaaaaaattt cctccaggtc atgtgatagt ggaaagtgga aatataattt
     1261 cttttgtggg ttatattaac tctggatgct gtaacattta tagaaatatt ataggatttg
     1321 tgaaactacg atcaaataaa gtgaaaagat ctcaaaaact tgtttatatg gggaaactta
     1381 aggagaagga gtcctttggt gagattagcg tccttcttca agttcctttc acgtgcacaa
     1441 tcattaccaa aaaagaagtt gagatggcaa tcattgaaga taaggaccta tttgtggcct
     1501 gattgccaac tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
     1561 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
     1621 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
     1681 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
     1741 gccaggacag aaatgcctcg acttcgctgc tacccaaggt tgccgggtga cgcacaccgt
     1801 ggaaacggat gaaggcacga acccagtgga cataagcctg ttcggttcgt aagctgtaat
     1861 gcaagtagcg tatgcgctca cgcaactggt ccagaacctt gaccgaacgc agcggtggta
     1921 acggcgcagt ggcggttttc atggcttgtt atgactgttt ttttggggta cagtctatgc
     1981 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     2041 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatt atgagggaag
     2101 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
     2161 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
     2221 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
     2281 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
     2341 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
     2401 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
     2461 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
     2521 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
     2581 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
     2641 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
     2701 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
     2761 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
     2821 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
     2881 aaccctcgag ccacccatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
     2941 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3001 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3061 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3121 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3181 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3241 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3301 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3361 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3421 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3481 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     3541 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     3601 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     3661 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     3721 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     3781 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     3841 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     3901 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     3961 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     4021 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     4081 agcctttcgt tttatttgat gcct
//
