LOCUS       sequence-168112-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GCaMP6f knock in construct into the AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pAAVS1-PC-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    40..65
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    72..105
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    227..728
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 gacttcctct gccctctccg ctgccagatc tctcgaggcc ctgtgggagg aagagaagag
       61 gtcagaagct tataacttcg tataatgtat gctatacgaa gttattgccc cactgtgggg
      121 tggaggggac agataaaagt acccagaacc agagccacat taaccggccc tgggaatata
      181 aggtggtccc agctcgggga cacaggatcc ctggaggcag caaacatgct gtcctgaagt
      241 ggacataggg gcccgggttg gaggaagaag actagctgag ctctcggacc cctggaagat
      301 gccatgacag ggggctggaa gagctagcac agactagaga ggtaaggggg gtaggggagc
      361 tgcccaaatg aaaggagtga gaggtgaccc gaatccacag gagaacgggg tgtccaggca
      421 aagaaagcaa gaggatggag aggtggctaa agccagggag acggggtact ttggggttgt
      481 ccagaaaaac ggtgatgatg caggcctaca agaaggggag gcgggacgca agggagacat
      541 ccgtcggaga aggccatcct aagaaacgag agatggcaca ggccccagaa ggagaaggaa
      601 aagggaaccc agcgagtgaa gacggcatgg ggttgggtga gggaggagag atgcccggag
      661 aggacccaga cacggggagg atccgctcag aggacatcac gtggtgcagc gccgagaagg
      721 aagtgctccg gaaagagcat ccttgggcag caacacagca gagagcaagg ggaagaggga
      781 gtggaggaag acggaacctg aaggaggcgg cagggaagga tctgggccag ccgtagaggt
      841 gacccaggcc acaagctgca gacaga
//
