LOCUS       sequence-168113-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a Fluorescent Reporter for Hydrogen Sulfide in Mammalian 
            Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Chen ZJ, Ai HW
  TITLE     A highly responsive and selective fluorescent probe for imaging 
            physiological hydrogen sulfide.
  JOURNAL   Biochemistry. 2014 Sep 23;53(37):5966-74. doi: 10.1021/bi500830d. 
            Epub 2014 Sep 5.
  PUBMED    25141269
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bi500830d";
            journalName: "Biochemistry"; date: "2014-09-23- 23"; volume: "53"; 
            issue: "37"; pages: "5966-74"
COMMENT     Sequence Label: pcDNA3-hsGFP
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     328..349
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(461..482)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             854..871
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agcttgccgc caccatgggc tccagcccgt acaacagcca
      121 caaggtctat atcaccgccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
      181 ccacaacgtg gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
      241 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgtgctgag
      301 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
      361 gatcactctc ggcatggacg agctgtacaa ggtggacggc ggatccggtg gcaccggagt
      421 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      481 cgtaaacggc cacaagttca gcgtgagggg cgaaggcgag ggcgatgcca ccaacggcaa
      541 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
      601 gaccaccttg acctagggcg tgcagtgctt ctcccgctac cccgaccaca tgaagcagca
      661 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
      721 ggacgacggc acctacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
      781 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
      841 ggagtacaac tggcaccatc accatcacca ttaactcgag gtcgacaatc aacc
//
