LOCUS       sequence-168114-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GCaMP6f knock in construct into the AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pAAVS1-PC-GCaMP6f
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..69
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     282..303
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(409..430)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(670..689)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gttgactcat cacgtcgtaa gtggaataag acaggtcacg cagtcagagc tataggtcgg
       61 ctgagctcac tcgagaacgt ctatatcaag gccgacaagc agaagaacgg catcaaggcg
      121 aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccta ccactaccag
      181 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcgtg
      241 cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      301 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg taccggaggg
      361 agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
      421 gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg cgatgccacc
      481 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
      541 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
      601 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acatccagga gcgcaccatc
      661 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
      721 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
      781 cacaagctgg agtacaacct gccggaccaa ctgactgaag agcagatcgc agaatttaaa
      841 gaggaattct ccctatttga caaggacggg gatgggacaa taacaaccaa ggagctgggg
      901 acggtgatgc ggtctctggg gcagaacccc acagaagcag agctgcagga catgatcaat
      961 gaagtagatg ccgacg
//
