LOCUS       sequence-168118-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Tang ED, Nunez G, Barr FG, Guan KL
  TITLE     Negative regulation of the forkhead transcription factor FKHR by 
            Akt.
  JOURNAL   J Biol Chem. 1999 Jun 11. 274(24):16741-6.
  PUBMED    10358014
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Jun 11. 274(24):16741-6."
COMMENT     Sequence Label: pcDNA3 HA human NEMO
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             94..120
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             100..126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca gcttaccatg gcctacccct acgacgtgcc cgactacgcc
      121 tccctcggat ccatgaatag gcacctctgg aagagccaac tgtgtgagat ggtgcagccc
      181 agtggtggcc cggcagcaga tcaggacgta ctgggcgaag agtctcctct ggggaagcca
      241 gccatgctgc acctgccttc agaacagggc gctcctgaga ccctccagcg ctgcctggag
      301 gagaatcaag agctccgaga tgccatccgg cagagcaacc agattctgcg ggagcgctgc
      361 gaggagcttc tgcatttcca agccagccag agggaggaga aggagttcct catgtgcaag
      421 ttccaggagg ccaggaaact ggtggagaga ctcggcctgg agaagctcga tctgaagagg
      481 cagaaggagc aggctctgcg ggaggtggag cacctgaaga gatgccagca gcagatggct
      541 gaggacaagg cctctgtgaa agcccaggtg acgtccttgc tcggggagct gcaggagagc
      601 cagagtcgct tggaggctgc cactaaggaa tgccaggctc tggagggtcg ggcccgggcg
      661 gccagcgagc aggcgcggca gctggagagt gagcgcgagg cgctgcagca gcagcacagc
      721 gtgcaggtgg accagctgcg catgcagggc cagagcgtgg aggccgcgct ccgcatggag
      781 cgccaggccg cctcggagga gaagaggaag ctggcccagt tgcaggtggc ctatcaccag
      841 ctcttccaag aatacgacaa ccacatca
//
