LOCUS       sequence-168124-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dox-inducible CRISPR interference (CRISPRi) knock in construct into 
            the AAVS1 locus with mCherry marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan
            AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen 
            TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, 
            Weissman JS, Qi LS, So PL, Conklin BR
  TITLE     CRISPR Interference Efficiently Induces Specific and Reversible Gene
            Silencing in Human iPSCs.
  JOURNAL   Cell Stem Cell. 2016 Apr 7;18(4):541-53. doi: 
            10.1016/j.stem.2016.01.022. Epub 2016 Mar 10.
  PUBMED    26971820
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.01.022"; journalName: "Cell Stem Cell"; date: 
            "2016-04-7- 7"; volume: "18"; issue: "4"; pages: "541-53"
COMMENT     Sequence Label: pAAVS1-NDi-CRISPRi (Gen1)
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agagcaaagt gatcaacagc gccctggaac tgctgaacgg cgtgggcatc gagggcctga
       61 ccacccggaa gctggcccag aaactgggcg tggaacagcc caccctgtac tggcacgtga
      121 agaacaagcg ggccctgctg gacgccctgc ccatcgagat gctggaccgg caccacaccc
      181 acagctgccc tctggaaggc gagagctggc aggacttcct gcggaacaac gccaagagct
      241 acagatgcgc cctgctgagc caccgggacg gcgccaaagt gcacctgggc accagaccca
      301 ccgagaagca gtacgagaca ctggaaaacc agctggcctt cctgtgccag cagggcttca
      361 gcctggaaaa cgccctgtac gccctgagcg ccgtgggcca cttcaccctg ggctgcgtgc
      421 tggaagaaca ggaacaccag gtcgccaaag aggaaagaga gacacccacc accgacagca
      481 tgccccccct gctgaagcag gccatcgagc tgttcgacag acagggcgcc gagcccgcct
      541 tcctgttcgg cctggaactg atcatctgcg gcctcgagaa gcagctgaag tgcgagagcg
      601 gcggacccac cgacgccctg gacgacttcg acctggacat gctgcccgcc gatgccctgg
      661 atgattttga tctggatatg ctccctgctg acgctctcga tgacttcgat ctcgatatgc
      721 tgccaggcta aaacgcgtga attcactcct caggtgcagg ctgcctatca gaaggtggtg
      781 gctggtgtgg ccaatgccct ggctcacaaa taccactgag atct
//
