LOCUS       sequence-168131-        4029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ADORA2A , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ADORA2A_p.R220W
FEATURES             Location/Qualifiers
     source          1..4029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1429..1528)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1545..1564)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1546..1564)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1569..1585)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1569..1585)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2016..2807
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2900..3488
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3389..3408
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3642..3659
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3818..3845)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3937..4023)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgcccatc atgggctcct cggtgtacat cacggtggag ctggccattg
      241 ctgtgctggc catcctgggc aatgtgctgg tgtgctgggc cgtgtggctc aacagcaacc
      301 tgcagaacgt caccaactac tttgtggtgt cactggcggc ggccgacatc gcagtgggtg
      361 tgctcgccat cccctttgcc atcaccatca gcaccgggtt ctgcgctgcc tgccacggct
      421 gcctcttcat tgcctgcttc gtcctggtcc tcacgcagag ctccatcttc agtctcctgg
      481 ccatcgccat tgaccgctac attgccatcc gcatcccgct ccggtacaat ggcttggtga
      541 ccggcacgag ggctaagggc atcattgcca tctgctgggt gctgtcgttt gccatcggcc
      601 tgactcccat gctaggttgg aacaactgcg gtcagccaaa ggagggcaag aaccactccc
      661 agggctgcgg ggagggccaa gtggcctgtc tctttgagga tgtggtcccc atgaactaca
      721 tggtgtactt caacttcttt gcctgtgtgc tggtgcccct gctgctcatg ctgggtgtct
      781 atttgcggat cttcctggcg gcgcgacgac agctgaagca gatggagagc cagcctctgc
      841 cgggggagtg ggcacggtcc acactgcaga aggaggtcca tgctgccaag tcactggcca
      901 tcattgtggg gctctttgcc ctctgctggc tgcccctaca catcatcaac tgcttcactt
      961 tcttctgccc cgactgcagc cacgcccctc tctggctcat gtacctggcc atcgtcctct
     1021 cccacaccaa ttcggttgtg aatcccttca tctacgccta ccgtatccgc gagttccgcc
     1081 agaccttccg caagatcatt cgcagccacg tcctgaggca gcaagaacct ttcaaggcag
     1141 ctggcaccag tgcccgggtc ttggcagctc atggcagtga cggagagcag gtcagcctcc
     1201 gtctcaacgg ccacccgcca ggagtgtggg ccaacggcag tgctccccac cctgagcgga
     1261 ggcccaatgg ctacgccctg gggctggtga gtggagggag tgcccaagag tcccagggga
     1321 acacgggcct cccagacgtg gagctcctta gccatgagct caagggagtg tgcccagagc
     1381 cccctggcct agatgacccc ctggcccagg atggagcagg agtgtcctgc ccaactttct
     1441 tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac gaacaggtca
     1501 ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat agtgagtcgt
     1561 attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt
     1621 acattgcaca agataaaaat atatcatcat gcctcctcta gaccagccag gacagaaatg
     1681 cctcgacttc gctgctaccc aaggttgccg ggtgacgcac accgtggaaa cggatgaagg
     1741 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     1801 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     1861 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     1921 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     1981 agcagggcag tcgccctaaa acaaagttaa acattatgag ggaagcggtg atcgccgaag
     2041 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     2101 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     2161 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     2221 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     2281 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     2341 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     2401 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     2461 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     2521 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     2581 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     2641 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     2701 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     2761 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataaccc tcgagccacc
     2821 catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag
     2881 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2941 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     3001 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     3061 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     3121 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     3181 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     3241 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     3301 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     3361 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     3421 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     3481 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     3541 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     3601 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     3661 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     3721 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata
     3781 cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     3841 cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     3901 ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     3961 caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat
     4021 ttgatgcct
//
