LOCUS       sequence-168136-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Dual Fluorescent endogenous miRNA sensor. Perfect complementary 
            region of an siRNA control targeting firefly luciferase encoded by 
            the pGL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Lemus-Diaz N, Boker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M,
            Gruber J
  TITLE     Dissecting miRNA gene repression on single cell level with an 
            advanced fluorescent reporter system.
  JOURNAL   Sci Rep. 2017 Mar 24;7:45197. doi: 10.1038/srep45197.
  PUBMED    28338079
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-03-24"; volume: "7:45197. doi"; pages: " 
            10.1038/srep45197"
COMMENT     Sequence Label: p.UTA.2.0-GL-2
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(82..84,85..87,88..801)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(127..148)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(388..407)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     735..756
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    841..962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(852..871)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     906..925
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cgagcgaccc tgcagcgacc cgcttaaaag cttggcattc cggtactgtt ggtaaagccc
       61 taagaacatt aagaagggcc catggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca gtgcttcgcc
      301 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      781 atggacgagc tgtacaagtg atctagagtc ggggcggccg gccgcttcga gcagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttaacaacaa caattgcatt cattttatgt ttcaggttc
//
