LOCUS       sequence-168137-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a Fluorescent Reporter for Peroxynitrite in Mammalian Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Chen ZJ, Ren W, Wright QE, Ai HW
  TITLE     Genetically encoded fluorescent probe for the selective detection of
            peroxynitrite.
  JOURNAL   J Am Chem Soc. 2013 Oct 9;135(40):14940-3. doi: 10.1021/ja408011q. 
            Epub 2013 Sep 26.
  PUBMED    24059533
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja408011q";
            journalName: "J Am Chem Soc"; date: "2013-10-9- 9"; volume: "135"; 
            issue: "40"; pages: "14940-3"
COMMENT     Sequence Label: pcDNA3-pnGFP
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      88..97
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     327..348
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(460..481)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             853..870
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gcttgccgcc accatgggct ccagcactta caacagccac
      121 aaggtctata tcaccgccga caagcagaag aacggcatca aggtgaactt caagatccgc
      181 cacaacgtgg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      241 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgtgctgagc
      301 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      361 atcactctcg gcatggacga gctgtacaag gtggacggcg gatccggtgg caccggagtg
      421 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
      481 gtaaacggcc acaagttcag cgtgaggggc gaaggcgagg gcgatgccac caacggcaag
      541 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      601 accaccttga cctagggcgt gcagtgcttc tcccgctacc ccgaccacat gaagcagcac
      661 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
      721 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      781 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      841 gagtacaaca cgcaccatca ccatcaccat taactcgagg tcg
//
