LOCUS       sequence-168142-        7249 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human FOXP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 7249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_FOXP1
FEATURES             Location/Qualifiers
     source          1..7249
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             837..860
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             864..887
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             891..914
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             930..986
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             999..1793
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1053..1072)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1663..1682
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2751..2767)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2760..2782)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(2986..3801)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3709..3728
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3793..3812
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      4181..4769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4670..4689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4923..4940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5240..5261
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5276..5281,5282..5299,5300..5306)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5314..5330
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5319..5341
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5338..5354
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5338..5354
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5531..5733
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5684..5704
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5730..5754
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5830..6960
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
ORIGIN
        1 aaagcagaac acacagcaag ccatgattag cccagcctcc tctgctcagt ggtccccaca
       61 aactgccgtt gcattacaga ttcttatttc gtgtaattat ttgaagatgg gtattcatcc
      121 acccaggatg ccactacggg acctgttatc tggtactcag tgtggttcta ggaccaccgg
      181 catcacttgg gggcttgtta gagatgcaga gtctcaggcc ccaccccaga cctgaatcac
      241 acactgcatt tcaacaaggc ccacaggtga ctgcagcgtg tgttaaagta tgagaaatgc
      301 caccagggag gagaggccca caccagtcca gagactagtg attcagagca tcactaccca
      361 agagaacttt acgcagtgat gcaaatgttc cgtctgtctc tgtgctggcc agaattaacc
      421 gctagtcaca tggggccttt gaacataaat gtggctagca caactcagaa actgaatatt
      481 aaatgtagta agatttcgtt catttcaatt taaagagcca cgtgtggcta atggtaacaa
      541 tactggacag agttctagtt ctagagtgcg gcttttctgt tgccccaaac ttttcagtgc
      601 acctgagctg caatgggctg ggaagtaccc tcactgaacc tctgcatatt ttttccaggc
      661 atcctgtaca cgtcaaagaa gagcccctcg atccagagga agctgaaggg cccctgtcct
      721 tagtgacaac agccaaccac agtccagatt ttgaccatga cagagattac gaagatgaac
      781 cagtaaacga ggacatggag gggagcggag gaggttccgg tggaggtggt tctggagatt
      841 acaaggatga cgacgataag ggcgattaca aggatgacga cgataaggga gattacaagg
      901 atgacgacga taaggtttca ggaagcggag ctactaactt cagcctgctg aagcaggctg
      961 gagacgtgga ggagaaccct ggacctggat cgtttcgcat gattgaacaa gatggattgc
     1021 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     1081 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     1141 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     1201 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     1261 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     1321 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     1381 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     1441 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     1501 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     1561 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     1621 actgtggccg gctgggtgtg gccgaccgct atcaggacat agcgttggct acccgtgata
     1681 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     1741 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgattcgaac
     1801 atcccaaccc cgagaatgaa gattggaaaa aggaaaaaaa aaaacacgtc aaaagttagc
     1861 agtgaaattg ttctccattt gttgtacagt ctggaggatt ttcactacgt tttgacaact
     1921 ctgaaatgtg ttaactctta gtgccatcaa aaaccccatt tgggagtatt tttgattttt
     1981 ctactttttg ttgaaaaaag gaatttgtac tctgtgcatt ggatggactt gtttggtact
     2041 tgggattttc ctctcttaac cgtcaacatc agtgttgtaa atttgctaaa ctgattcact
     2101 tttagcagca gactttgaac tgcagtcctg ccaacgttgg acactgagga cgcccgacag
     2161 agcttgtgca cctaagctgc agaccaagcc tttgcccaga atttaaggat tccaatggac
     2221 gacctatttg cacagtactg catgttgatt atcactgcct ttactccttt tttttttttt
     2281 tttttttttt ttttttgctt ccagttggga tggggaaggc ctttgtgtgt gtattggggg
     2341 gaggggttaa aaaataatta tcccaaactt tttaatgtat tgcttttttt tttttttttt
     2401 tccttctact ataccatttt aagttctgac ctcaggcctc catttgggcc gatggcctct
     2461 tggaggctta aagttttctg taccttgtga tgaatgttaa taggtgtttt tattatacaa
     2521 agctgaatgt catttctcgt ttgtagcttt ctgtcactca ttccatcttc cttcagacat
     2581 caccacgttt ctctaaagtc agaaaacatt ccgttttggt ctttttcaaa aaggtcccaa
     2641 atgctgcact ctacacatga aggccctctc acacagacgt gacgtcctgc cagaaagact
     2701 tggaaagtcc tctccactga ctgtagcctc caattcactg gccgtcgttc actggccgtc
     2761 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     2821 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     2881 cagttgcgca gcctgaatgg cgaatgcgat ttattcaaca aagccgccgt cccgtcaagt
     2941 cagcgtaatg ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc
     3001 gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa
     3061 aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc
     3121 ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc
     3181 gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa
     3241 tggcaaaagc ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc
     3301 atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg
     3361 aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag
     3421 gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg
     3481 gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat
     3541 aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc
     3601 atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc
     3661 gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca
     3721 tttataccca tataaatcag catccatgtt ggaatttaat cgcggcttcg agcaagacgt
     3781 ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag cagacagttt
     3841 tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     3901 aacgtggctt tgttgaataa atcgaacttt tgctgagttg aaggatcaga tcacgcatct
     3961 tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc aaaatcacca actggtccac
     4021 ctacaacaaa gctctcatca accgtggctc cctcactttc tggctggatg atggggcgat
     4081 tcaggcctgg tatgagtcag caacaccttc ttcacgaggc agacctctcg acggagttcc
     4141 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4201 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4261 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4321 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4381 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4441 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4501 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4561 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4621 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4681 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4741 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4801 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4861 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4921 gcagcgagtc agtgagcgag gaagcggaag aagctcgcac attcagcagc gtttttcagc
     4981 gcgttttcga tcaacgtttc aatgttggta tcaacaccag gtttaacttt gaacttatcg
     5041 gcactgacgg ttactgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg
     5101 aagatgcgtg atctgatcct tcaactcagc aaaagttccc aatacgcaaa ccgcctctcc
     5161 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     5221 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     5281 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     5341 gaaacagcta tgaccatgat tacgccaagc ttgcatgcct gcaggtcgac tctagaggat
     5401 ccccgggtac cgagctcgaa ttagcttgca tgcctgcagg tcaattccct ggcattatgc
     5461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     5521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     5581 acggggattt caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     5641 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     5701 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
     5761 agacgccatc cacgctgttt tgacctccat aggagacacc gggaccgatc cagcctgggg
     5821 atcccgacca tggcttcgta cccctgccat caacacgcgt ctgcgttcga ccaggctgcg
     5881 cgttctcgcg gccatagcaa ccgacgtacg gcgttgcgcc ctcgccggca gcaagaagcc
     5941 acggaagtcc gcctggagca gaaaatgccc acgctactgc gggtttatat agacggtcct
     6001 cacgggatgg ggaaaaccac caccacgcaa ctgctggtgg ccctgggttc gcgcgacgat
     6061 atcgtctacg tacccgagcc gatgacttac tggcaggtgc tgggggcttc cgagacaatc
     6121 gcgaacatct acaccacaca acaccgcctc gaccagggtg agatatcggc cggggacgcg
     6181 gcggtggtaa tgacaagcgc ccagataaca atgggcatgc cttatgccgt gaccgacgcc
     6241 gttctggctc ctcatatcgg gggggaggct gggagctcac atgccccgcc cccggccctc
     6301 accctcatct tcgaccgcca tcccatcgcc gccctcctgt gctacccggc cgcgcgatac
     6361 cttatgggca gcatgacccc ccaggccgtg ctggcgttcg tggccctcat cccgccgacc
     6421 ttgcccggca caaacatcgt gttgggggcc cttccggagg acagacacat cgaccgcctg
     6481 gccaaacgcc agcgccccgg cgagcggctt gacctggcta tgctggccgc gattcgccgc
     6541 gtttacgggc tgcttgccaa tacggtgcgg tatctgcagg gcggcgggtc gtggcgggag
     6601 gattggggac agctttcggg gacggccgtg ccgccccagg gtgccgagcc ccagagcaac
     6661 gcgggcccac gaccccatat cggggacacg ttatttaccc tgtttcgggc ccccgagttg
     6721 ctggccccca acggcgacct gtacaacgtg tttgcctggg ccttggacgt cttggccaaa
     6781 cgcctccgtc ccatgcacgt ctttatcctg gattacgacc aatcgcccgc cggctgccgg
     6841 gacgccctgc tgcaacttac ctccgggatg gtccagaccc acgtcaccac ccccggctcc
     6901 ataccgacga tctgcgacct ggcgcgcacg tttgcccggg agatggggga ggctaactga
     6961 ggatcccacg tcactattgt atactctata ttatactcta tgttatactc tgtaatccta
     7021 ctcaataaac gtgtcacgcc tgtgaaaccg tactaagtct cccgtgtatt cttatcacca
     7081 tcaggtgaca tcctcgccca ggctgtcaat catgccggta tcgattccag tagcaccggc
     7141 cccacgctga caacccactc ttgcagcgtt agcagcgccc ctcttaacaa gccgaccccc
     7201 accagcgtcg cggttactaa cactcctctc cccgacctgc agcccagct
//
