LOCUS       sequence-16816-e        1420 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L4819
FEATURES             Location/Qualifiers
     source          1..1420
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..67
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     51..67
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     509..528
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     complement(678..697)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(679..697)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(707..724)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(707..723)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(716..738)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gatacctcct aagggaacaa aagctggagc tccaccgcgg tggcggccgc tctagaacta
       61 gtggatccac cggttcatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt
      121 taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat
      181 aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat
      241 gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt
      301 ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc
      361 tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa
      421 ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag
      481 ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt
      541 acgatgccat tgggatatat caacggtggt atatccagtg atttttttct ccattttagc
      601 ttccttagct cctgaaaatc tcgccaagct tgcatgcctg caggtcgact ctagaggatc
      661 cccgggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
      721 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      781 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      841 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
      901 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
      961 cttcttccct tcctttctcg ccacgttcgc cggctttncc cgtcaagctc taaatcgggg
     1021 gcctccttta gggttcnatt tagngcttta cggcnccncn accccaaaaa cttgatnagg
     1081 gngagggtcn cgtnngggcc cccncccgga naaacgnttt tccccctttg acgtggngnc
     1141 ccgttcntta nagggaccct tgttccnann ggaaaacccc cacccttccg gcctntnttt
     1201 gannaangga ttngcnatnc gcccntggtt aaaaaagncc gattanaaat ttcnncattt
     1261 aaaaaatnac cctaantngg gnccttncgg aagggnngcn cccttntttt tttnaaantn
     1321 aangnccccc ggaaacccga ggnntaatag aagaaaaaaa aaatcngccc cccttttntt
     1381 ttcncttttc ccnncnggaa aaaaaantgg gggnttccnc
//
