LOCUS       sequence-168163-        6963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human ZNF792.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_ZNF792
FEATURES             Location/Qualifiers
     source          1..6963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             738..761
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             765..788
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             792..815
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             831..887
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             900..1694
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(954..973)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1564..1583
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2465..2481)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2474..2496)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(2700..3515)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     3423..3442
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     3507..3526
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3895..4483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4384..4403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4637..4654
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4954..4975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4990..4995,4996..5013,5014..5020)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5028..5044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5033..5055
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5052..5068
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5052..5068
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5245..5447
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5398..5418
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5444..5468
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             5544..6674
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
ORIGIN
        1 gtgggaaatc tttcaactgc aactccagcc taattaaaca ttggagagtt cacactggag
       61 aaagacctta caagtgtaac gaatgtggga aattcttcag ccacattgcc agcctcattc
      121 aacatcagat agttcacact ggcgagcggc ctcacgggtg tggtgagtgt gggaaagcct
      181 tcagccgaag ctctgacctc atgaaacatc agcgagttca cactggtgag cggccttatg
      241 aatgcaatga atgtgggaag ttatttagcc agagctccag cctcaatagc catcggagac
      301 ttcacactgg tgaacggcct taccagtgca gtgaatgtgg gaaattcttt aaccaaagct
      361 ccagcctcaa taaccaccgg agacttcaca ccggcgagcg gccttatgag tgcagcgaat
      421 gtgggaaaac cttcaggcag aggtccaatc tgaggcagca cctgaaagtt cacaaaccag
      481 acaggcctta cgaatgcagc gaatgtggga aagccttcaa ccaaaggcct accctcattc
      541 ggcatcagaa gattcacatc agagaaagga gcatggagaa tgtgctcctt ccctgttcac
      601 agcacacacc agagataagc tctgagaaca gaccttatca gggcgctgtc aactacaagt
      661 tgaaacttgt tcatccaagt acccaccctg gggaggttcc cgggagcgga ggaggttccg
      721 gtggaggtgg ttctggagat tacaaggatg acgacgataa gggcgattac aaggatgacg
      781 acgataaggg agattacaag gatgacgacg ataaggtttc aggaagcgga gctactaact
      841 tcagcctgct gaagcaggct ggagacgtgg aggagaaccc tggacctgga tcgtttcgca
      901 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      961 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     1021 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     1081 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     1141 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     1201 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     1261 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     1321 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     1381 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     1441 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     1501 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggccgaccgc tatcaggaca
     1561 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1621 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1681 acgagttctt ctgattcgaa catcaatgct agctgtgttg gaagctttct ggagacaagt
     1741 tacattctct tactgtagag tttatcagcg ttttttcact gctggggttg tgatagaagc
     1801 catgtcagca ccacacactg cagctctcca aagagtgtgt ccaccactcc actgtgctca
     1861 gggaaggcag acctctcctc tctctttcca atccctaaag ggaattagga gtagtctgaa
     1921 gccttgggaa gatgtcattc ccgccctgta tggctggttt agccatggac atgaccagct
     1981 tttggctgtg aagacctgag cagggttttg ccaacagctt gttctagaga aggtttccct
     2041 ttttctggga cattgttggt ctcatgttat gcctgtgaag gatttgtcca gccttcatgt
     2101 tactctacac aacaacttat ctcctgtgta ttgccctggt tcatgtgatg ataatggcct
     2161 tatgataaag ccgcctttag tcttccatgt tctgatcact gtgtggggtg gttcaagagc
     2221 agagttaaga tctaccaaac tgaaggggtc tccagattat cttggagggg acaggaggat
     2281 ggcagagaaa caactgtcaa tccagatttg acctcatttg cattgccacc aaggcctcct
     2341 aggaaaaatt gtaggaattt atgggtataa tattgtggtc tgaatggcgt gaatttttat
     2401 cccgaggagg ggcttggaaa gtcctctcca ctgactgtag cctccaattc actggccgtc
     2461 gttcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2521 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2581 atcgcccttc ccaacagttg cgcagcctga atggcgaatg cgatttattc aacaaagccg
     2641 ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat
     2701 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     2761 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     2821 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     2881 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     2941 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
     3001 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     3061 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     3121 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     3181 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     3241 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     3301 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     3361 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     3421 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     3481 ttcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     3541 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     3601 agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga gttgaaggat
     3661 cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc
     3721 accaactggt ccacctacaa caaagctctc atcaaccgtg gctccctcac tttctggctg
     3781 gatgatgggg cgattcaggc ctggtatgag tcagcaacac cttcttcacg aggcagacct
     3841 ctcgacggag ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3901 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3961 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     4021 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     4081 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     4141 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     4201 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     4261 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     4321 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     4381 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4441 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4501 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     4561 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     4621 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagaagctc gcacattcag
     4681 cagcgttttt cagcgcgttt tcgatcaacg tttcaatgtt ggtatcaaca ccaggtttaa
     4741 ctttgaactt atcggcactg acggttactg attttgaact tttgctttgc cacggaacgg
     4801 tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcccaatacg
     4861 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4921 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     4981 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     5041 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcaggt
     5101 cgactctaga ggatccccgg gtaccgagct cgaattagct tgcatgcctg caggtcaatt
     5161 ccctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     5221 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     5281 tagcggtttg actcacgggg atttcaagtc tccaccccat tgacgtcaat gggagtttgt
     5341 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     5401 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     5461 gtcagatcgc ctggagacgc catccacgct gttttgacct ccataggaga caccgggacc
     5521 gatccagcct ggggatcccg accatggctt cgtacccctg ccatcaacac gcgtctgcgt
     5581 tcgaccaggc tgcgcgttct cgcggccata gcaaccgacg tacggcgttg cgccctcgcc
     5641 ggcagcaaga agccacggaa gtccgcctgg agcagaaaat gcccacgcta ctgcgggttt
     5701 atatagacgg tcctcacggg atggggaaaa ccaccaccac gcaactgctg gtggccctgg
     5761 gttcgcgcga cgatatcgtc tacgtacccg agccgatgac ttactggcag gtgctggggg
     5821 cttccgagac aatcgcgaac atctacacca cacaacaccg cctcgaccag ggtgagatat
     5881 cggccgggga cgcggcggtg gtaatgacaa gcgcccagat aacaatgggc atgccttatg
     5941 ccgtgaccga cgccgttctg gctcctcata tcggggggga ggctgggagc tcacatgccc
     6001 cgcccccggc cctcaccctc atcttcgacc gccatcccat cgccgccctc ctgtgctacc
     6061 cggccgcgcg ataccttatg ggcagcatga ccccccaggc cgtgctggcg ttcgtggccc
     6121 tcatcccgcc gaccttgccc ggcacaaaca tcgtgttggg ggcccttccg gaggacagac
     6181 acatcgaccg cctggccaaa cgccagcgcc ccggcgagcg gcttgacctg gctatgctgg
     6241 ccgcgattcg ccgcgtttac gggctgcttg ccaatacggt gcggtatctg cagggcggcg
     6301 ggtcgtggcg ggaggattgg ggacagcttt cggggacggc cgtgccgccc cagggtgccg
     6361 agccccagag caacgcgggc ccacgacccc atatcgggga cacgttattt accctgtttc
     6421 gggcccccga gttgctggcc cccaacggcg acctgtacaa cgtgtttgcc tgggccttgg
     6481 acgtcttggc caaacgcctc cgtcccatgc acgtctttat cctggattac gaccaatcgc
     6541 ccgccggctg ccgggacgcc ctgctgcaac ttacctccgg gatggtccag acccacgtca
     6601 ccacccccgg ctccataccg acgatctgcg acctggcgcg cacgtttgcc cgggagatgg
     6661 gggaggctaa ctgaggatcc cacgtcacta ttgtatactc tatattatac tctatgttat
     6721 actctgtaat cctactcaat aaacgtgtca cgcctgtgaa accgtactaa gtctcccgtg
     6781 tattcttatc accatcaggt gacatcctcg cccaggctgt caatcatgcc ggtatcgatt
     6841 ccagtagcac cggccccacg ctgacaaccc actcttgcag cgttagcagc gcccctctta
     6901 acaagccgac ccccaccagc gtcgcggtta ctaacactcc tctccccgac ctgcagccca
     6961 gct
//
