LOCUS       sequence-16819-e        1353 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBK125
FEATURES             Location/Qualifiers
     source          1..1353
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             456..476
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(519..547)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gngctgcggt cgactctaga ggatcaccaa aaacggaacg ttgagctgga cggaaatagt
       61 ggtaaagtga catgattata gtttgaagat ctctaatttc acaattagag caaatgttgt
      121 tcggtattta ttttcaacgg tatttatact attttccacc tttttctaga acattcgagc
      181 tgcttgttgc aaaaggaggg cgactcacat tcggtacatg gaaaagtagt gtacacaata
      241 aagagaccca gatacatttt ccgtctgcgt ctctttgcac ccaccgggag tattttcaaa
      301 cgaatgcatc taggaccttc tagaacattc tgtaaggctg cagaatgcgg gtatataagg
      361 aaagcgggct cagaggaagc caacacgctt tgttctagtg catctaaaaa acttcgaaaa
      421 tcctcatcgg atccccctca gaaaaaatga ctgctccaaa gaagaagcgt aaggtaccgg
      481 tagaaaaaat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      541 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
      601 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      661 caacacttgt cactactttc tgttatggtg ttcaatgctt ctcgagatac ccagatcata
      721 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta
      781 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata
      841 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcntg
      901 gacacnaatt ggaatacaac tataactcac acaatgtata cntcatggca gacaaacaaa
      961 gaatggaatc caagttactt caaattagac ncacattgaa gatgnagcgt ccactagcan
     1021 accttatcca caaatactcc attggcgang gcctgtcntt tacnnacacc ttactgtcnc
     1081 ncatctgncc nttcgaagat ccacgaaaaa anaccnnngg cctctgattg naccncgcgg
     1141 gatacctggc ggagacnaca anacntngaa ntccctgngn cggccnctta cantggnggg
     1201 caaaaaaggc cggtaaannc cccgccggcc ngattaanna tactnancca ngnttncggg
     1261 cccccnttnt taaaannctn tttnttttct tnccccnngg nggnanaaaa ttnaaannaa
     1321 ngcccccnaa aaccccngng ncttttttnt taa
//
