LOCUS       sequence-16821-e        1433 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLT61
FEATURES             Location/Qualifiers
     source          1..1433
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..52
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     37..53
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(821..837)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(821..837)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(894..913)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(895..913)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(937..954)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aatcctatag ggcgaattgg gtaccgggcc ccccctcgag gtcgacggta tcgataagct
       61 tgatatccaa ttcggaagat gggttccatg cgccaaagtt aaggatacna aggctcatat
      121 tgatggtctc aagaagggac aaacatatca gttccgtgtc aaggctgtca ataaggaagg
      181 agcttctgat gcattgtcta ctgataagga caccaaagcc aagaatccat atgatgagcc
      241 aggaaaaacc ggaactccgg atgttgtcga ctgggatgct gatcgtgtct cacttgaatg
      301 ggaaccacca aagtctgacg gaggagctcc aatcactcaa tacgtcattg agaagaaggg
      361 caaacatgga agagactggc aagaatgcgg aaaggtttct ggagatcaaa ccaatgctga
      421 gattcttgga ctcaaggagg gagaagagta ccagttccgt gtgaaggctg ttaacaaggc
      481 cggaccggga gaggcttcag acccaagccg aaaggttgtt gcaaagccaa gaaacttgaa
      541 gccatggatt gatcgtgaag caatgaagac gatcactatc aaggtaggaa acgatgtgga
      601 attcgatgtt ccagtacgcg gagaaccacc accgaagaag gaatggatct tcaatgagaa
      661 accagtcgat gatcaaaaga tcaggattga aagcgaagac tacaagaccc gatttgtgct
      721 ccgtggagca actcgcaagc atgctggttt gtacactctt actgctacca acgcttctgg
      781 aagcgacaaa cattccgttg aggtcgttgt gctcgggggg gatccactag ttctagagcg
      841 gccgccaccg cggtggagct cgaattcatc gatgatatca gatctgccgg tctccctata
      901 gtgagtcgta ttaatttcga taagccaggt tgcttcctcg ctcactgact cgctgcgctc
      961 ggtcgttcgg ctgcggcgag cggtatcagc tcnctcaagg cggtaatacg gttatcccca
     1021 gaatcagggg ataaccccag naanaacatg tgaacaaaan ggcccncaaa aggccngnaa
     1081 ccgtaaaaag gcccgttgct ggggtttttc caangccccc cccccctgac nacctcncaa
     1141 antcnacnct ccagtcgnng ggggnaaacc cnacngaann aaananccag gggtttcccc
     1201 gggaaacncc ncggnnccnc ctgttccanc ngnccntacg gaaactgncc ccnttnccct
     1261 cgganggggg ccttnccaac ncccngnana atcnntnggg gaggcctccc ccccgggggg
     1321 ggnnacccct tnccccccgg cctttggaat ttntttnccc ggaaanantt ccnncccccg
     1381 taanaaaaaa gggggggccc ctttnnggcc ccccaaaaan tttttcccnc nct
//
