LOCUS       sequence-16822-e        1387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1999
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLT63
FEATURES             Location/Qualifiers
     source          1..1387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..44
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     29..45
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(729..745)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(729..745)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(802..821)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(803..821)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(845..862)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ataggggaat tgggtacggg ccccccctcg aggtcgacgg tatcgataag cttgataccn
       61 nntnggngcg ctattattca ttaaattcca ttctccttan ccaatttctt caatttctgg
      121 ctctctccgt ggtataatga agttattcgc gttccaaata atctaataac tctcaatttc
      181 tgattcgaca cgcacctagt ggtagatcat caaagttggc gtgattcaac gctttcatac
      241 gtttcatcac attcaacacc acatctccgt gccgattcct tgcgaacagc catgtgccac
      301 gtacgagcag tgctcgaacc aagcttttcc taaactctct ggctcgaagc agaatgtgca
      361 ttggagtgtc gttattctta tcagtggcat tcacatctag gtcaagttct agaagttgtt
      421 ccaaaactgc agctttggga agtcccaact ctgcactgtt actatcagga atcctctctt
      481 caagtgtatg atgtagaagt ggaatatgta attcatggca cacgatcatc agtcttcgaa
      541 cgtcggcttt cggctctttt tcctcatcgg aatcatcgat atgtgcagaa ggaagagata
      601 gtcgttcgag tacattcaca agatgcacag ctacatatac aagtttcttg tattcagatt
      661 cttcagtcgc atgctgacat ctctcttttc cacagcattc cgtatcctct aatagtggag
      721 caccggtgga tccactagtt ctagagcggc cgccaccgcg gtggagctcg aattcatcga
      781 tgatatcaga tctgccggtc tccctatagt gagtcgtatt aatttcgata agccaggttg
      841 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      901 actcaaggcg gtaatacggt tatccacaga atcaggggat ancgcaggaa agaacatgtg
      961 agcaaaaggc nagcaaaagg gccaggaaac cgnaaaangg ccgcgtggct ggggnttttt
     1021 nccanaggct cngcccccct gacgagcatc acaaaaatcg acgctcaagt cagaaggngg
     1081 cgaaaccnga caggactata aaaaaccagg gnttccccct ggnaancncc cncgggggcc
     1141 cnctggtncc anccnggcgc taacngaaac cggncccctt tttcccttnn gggagggggg
     1201 gcnttcccaa ncccncnggg angatnccat ggggggnggc nnccccnagg ggggggggga
     1261 aaanncccnt tacccggggg gccttnaggn aaatngtngg accccggaaa aaanntcccg
     1321 ggnccccggg aaaaanaaaa aggggggggg ccaatttgng gggcccccnc naaaaaaatg
     1381 tggccgc
//
