LOCUS       sequence-16824-e        1264 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1264)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD177.53
FEATURES             Location/Qualifiers
     source          1..1264
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             476..496
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(570..589)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gcgcngcngt cgactctaga ggatccccgg gtacctaatt tgatcgataa tatgtacata
       61 ttttgttatt cgttccggta tcgtacctcc acctaccata aatcccatac cctacatctt
      121 tatcgatttt ttgcaaatta cgagcgttgt agggggcgga gcgataggtc ctataggttt
      181 tggtatatca tcattcattc attcattggt acattcattt acccaccttc ctctttctga
      241 gcttctctgg agttctgtgc ttcctttttc ccttatcttt atactgtaat ttttaacttt
      301 caggcattga ttggatcccc gggattggcc aaaggaccca aaggtatgtt tcgaatgata
      361 ctaacataac atagaacatt ttcaggagga cccttggcta gaggatcccc gggattggcc
      421 aaaggatcca aaggaggacc cttggagggt accgagctca gaaaaaatga ctgctccaaa
      481 gaagaagcgt aaggtaccgg ctagattaga taaaagtaaa gtgattaaca gcgcattaga
      541 gctacttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg
      601 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt
      661 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg
      721 gcaagatttt ttacgtaagt ttaaacgtcg cagtactaac taacgtcttg gatttaaatt
      781 ttcaggtaat aacgctaaaa gttttagatg tgctttacta agtcatcgcg atggagcaaa
      841 agtacattta ggtacacggc ctacagaaaa acagtatgaa actctcgaaa atcaattagc
      901 ctttttatgc caacaaggtt tttcactaga gaatgcatta tatgcactca gcgctgtggg
      961 gcattttact ttaggtngcg tattggaaga tcaagagcat caagtcgcta aagangaang
     1021 gnaacnccta ctactgatag tatgccgccn ttattacgac agctatcgaa ttatttgatc
     1081 ncngtaagtt taaacgngga aaactaccta nccgncagta tttaattttc naagggncna
     1141 nccngcccnc ttatcggcct gaatgatcaa tggggataaa aaacactnaa ngggaanggg
     1201 gtccnaaaaa aagnaaaaan ttttccggga gtgnccnatt cngnggaata aagggnggtt
     1261 aggg
//
