LOCUS       sequence-168248-        4682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  membrane targeted Tfp1 expression in zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Yoshimatsu T, D'Orazi FD, Gamlin CR, Suzuki SC, Suli A, Kimelman D, 
            Raible DW, Wong RO
  TITLE     Presynaptic partner selection during retinal circuit reassembly 
            varies with timing of neuronal regeneration in vivo.
  JOURNAL   Nat Commun. 2016 Feb 3;7:10590. doi: 10.1038/ncomms10590.
  PUBMED    26838932
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-02-3"; volume: "7:10590. doi"; pages: " 
            10.1038/ncomms10590"
COMMENT     Sequence Label: pUAS-MmTFP13xmyc
FEATURES             Location/Qualifiers
     source          1..4682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             474..1181
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             1191..1220
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1227..1256
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1263..1292
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1430..1551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1467..1486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1521..1540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1558..2013)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1695..1716)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1907..1926
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2040..2144
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2146..2503
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2146..2337
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2169..2189)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2354..2489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2538..3332
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2538..3332
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2592..2611)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3202..3221
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3520..3539)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3564..3611
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3940..4528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4429..4448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcttaggcc tccaaggcgg agtactgtcc tccgggctgg cggagtactg tcctccggca
       61 aggtcggagt actgtcctcc gacactagag gtcggagtac tgtcctccga cgcaaggcgg
      121 agtactgtcc tccgggctgc ggagtactgt cctccggcaa ggtcggagta ctgtcctccg
      181 acactagagg tcggagtact gtcctccgac gcaaggtcgg agtactgtcc tccgacgcaa
      241 ggcggagtac tgtcctccgg gctggcggag tactgtcctc cggcaagggt cgactctaga
      301 gggtatataa tggatcccat cgcgtctcag cctcactttg agctcctcca cacgaattca
      361 cagccaccat gctgtgctgc atcagaagaa ctaaaccggt tgagaagaat gaagaggccg
      421 atcaggagct gcagtcgacg gtacctcaag cttgcggccg cctcgaggcc accatggtga
      481 gcaagggcga ggagaccaca atgggcgtaa tcaagcccga catgaagatc aagctgaaga
      541 tggagggcaa cgtgaatggc cacgccttcg tgatcgaggg cgagggcgag ggcaagccct
      601 acgacggcac caacaccatc aacctggagg tgaaggaggg agcccccctg cccttctcct
      661 acgacattct gaccaccgcg ttcgcctacg gcaacagggc cttcaccaag taccccgacg
      721 acatccccaa ctacttcaag cagtccttcc ccgagggcta ctcttgggag cgcaccatga
      781 ccttcgagga caagggcatc gtgaaggtga agtccgacat ctccatggag gaggactcct
      841 tcatctacga gatacacctc aagggcgaga acttcccccc caacggcccc gtgatgcaga
      901 agaagaccac cggctgggac gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga
      961 agggcgacgt caagcacaag ctgctgctgg agggcggcgg ccaccaccgc gttgacttca
     1021 agaccatcta cagggccaag aaggcggtga agctgcccga ctatcacttt gtggaccacc
     1081 gcatcgagat cctgaaccac gacaaggact acaacaaggt gaccgtttac gagagcgccg
     1141 tggcccgcaa ctccaccgac ggcatggacg agctgtacaa gagatcctta gagcagaaac
     1201 tcattagcga ggaggacctg aacagcgaac agaaactcat ttccgaagag gatctcaact
     1261 ccgagcagaa gctgatcagc gaggaggacc tgagatcctg agaattcgac gatatctcta
     1321 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     1381 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     1441 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1501 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     1561 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     1621 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     1681 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     1741 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     1801 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     1861 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     1921 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     1981 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     2041 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     2101 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     2161 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     2221 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     2281 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     2341 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     2401 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     2461 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     2521 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2581 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2641 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     2701 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     2761 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     2821 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     2881 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     2941 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3001 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3061 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3121 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3181 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3241 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3301 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3361 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3421 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3481 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     3541 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     3601 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     3661 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     3721 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
     3781 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     3841 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     3901 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3961 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4021 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4081 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4141 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4201 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4261 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4321 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4381 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4441 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4501 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4561 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     4621 taaccgtatt accgccatgc attagttatt actagcgcta ccggactcag atctcgagct
     4681 ca
//
