LOCUS       sequence-16825-e        1327 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1327)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 1327)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD107.09
FEATURES             Location/Qualifiers
     source          1..1327
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..72
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(142..158)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     600..616
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(686..702)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(727..746)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(728..746)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(756..773)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(756..772)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(765..787)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1004..1023)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggcattacct cactaaaggg aacaaaagct ggagctccac cgcggtggcg gccgctctag
       61 aactagtgga tccaccggtt ccatggctag ccacgtgacg cgtggatccc ccgggctgca
      121 ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg gtaccgcatt
      181 ttctcgccat tctcctcttt taaaaattga gagatccttt gagacggaga gaccgagagc
      241 actctccccc atttctcacc actctccctt ctcctactgc atgtcttcta tgtaaactgc
      301 atcctttcct cttgttttcg tgactgcttg gcgcaatggt agcgcgttcg actctagatc
      361 gaaaggttgg gcgttcgatc cgctcagtgg tcaaatgttt tgtttttgag ctttaagttt
      421 ggttctttca gttatggttt tttttgcaga tcacagtgtc aaataaagca agattgttcg
      481 cagagcggct caacaagcgt gataggaaag gaaagagtga aattgagaca tgccgacaag
      541 ctagatgtcc tccagaattc tggcaagcgc tcactgagct ccaccgcggt ggcggccgct
      601 ctagaactag tggatccacc ggttccatgg ctagccacgt gacgcgtgga tcccccgggc
      661 tgcaggaatt cgatatcaag cttatcgata ccgtcgacct cgaggggggg cccggtaccc
      721 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
      781 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      841 agctggcgta atagcgaana ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      901 aatggcgaat ggggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      961 ncncancgtg accgctacac ttggccagcg nccctancgc ccgctccttt cgctttcttc
     1021 ccttcctttc tcgccncgtt cnccggcttt ccccgtcaag ctctaaatcg ggggctccct
     1081 ttaagggtnc naattnangg ctttacggnn ncctcnaccc caaaaanttg ataaggggaa
     1141 nggtnccgna ngggncnacc ccctgaaaaa cggtttttcc ccnttnangt tggancccnn
     1201 tnnttaaaan ggaanctnnt tccaaangna aaaannnnna nccnanncng gnnntttttt
     1261 nantnaaagg gantttgccn attncnccnt tggntaaaaa nnnnnnnttn aaaantttnn
     1321 nnaattt
//
