LOCUS       sequence-168253-       11555 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter,
            also expresses mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11555)
  AUTHORS   Yoshimatsu T, D'Orazi FD, Gamlin CR, Suzuki SC, Suli A, Kimelman D, 
            Raible DW, Wong RO
  TITLE     Presynaptic partner selection during retinal circuit reassembly 
            varies with timing of neuronal regeneration in vivo.
  JOURNAL   Nat Commun. 2016 Feb 3;7:10590. doi: 10.1038/ncomms10590.
  PUBMED    26838932
REFERENCE   2  (bases 1 to 11555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-02-3"; volume: "7:10590. doi"; pages: " 
            10.1038/ncomms10590"
COMMENT     Sequence Label: pCx55.5-TrpR
FEATURES             Location/Qualifiers
     source          1..11555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(561..572,573..735)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     1108..1124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1144..1164
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     1166..1185
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1166..1184
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5790..5814
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6186..6527
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     protein_bind    complement(6598..6622)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6624..6758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(6821..6841)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6849..6865)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6881..7015
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(7151..7861)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7429..7448)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7698..7716
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    join(9085..9272,9273..9284)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9318..9334)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9318..9334)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9371..9391)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9371..9389)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9410..9426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9423..9445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9434..9450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9458..9464,9465..9482,9483..9488))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9503..9524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9641..9658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9812..10400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9892..9911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10571..11362,11363..11431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11194..11213
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11432..11536)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggccac aatgacggct actacatggt
      361 gccattcctt cctctttata ggaatggaga ctacctcctg tccaacaagg ctcttggata
      421 cgagtacgcc tacctgttgg acccaggtca ttgcacaaca ccagaaatgc cctctgatct
      481 gcaaaagacg tgaatatctg ttcagacacc catatccact ctgttccaca caggtcagag
      541 gtttgtccag gagttcttga cagaggtgta aaaagtactc aaaaatttta ctcaagtgaa
      601 agtacaagta cttagggaaa attttactca attaaaagta aaagtatctg gctagaatct
      661 tacttgagta aaagtaaaaa agtactccat taaaattgta cttgagtatt aaggaagtaa
      721 aagtaaaagc aagaaagaaa actagagatt cttgtttaag cttttaatct caaaaaacat
      781 taaatgaaat gcatacaagg ttttatcctg ctttagaact gtttgtattt aattatcaaa
      841 ctataagaca gacaatctaa tgccagtaca cgctactcaa agttgtaaaa cctcagattt
      901 aacttcagta gaagctgatt ctcaaaattg ttagtgtcaa gcctagctct tttggggctg
      961 aaaagcaatc ctgcagtgct gaaaagcctc tcacaggcag ccgatgcggg aagaggtgta
     1021 ttagtcttga tagagaggct gcaaatagca ggaaacgtga gcagagactc cctggtgtct
     1081 gaaacacagg ccagataggc cctcgagcag gaaacagcta tgaccatgat tacgccaagc
     1141 tatcaacttt gtatagaaaa gttggtaata cgactcacta tagggcgaat tgggtacaag
     1201 cgctaccgga ctcagatctc gagagatctt tccaacagca cacaataata cacttgatat
     1261 atatggcttt gagtaatcaa tgtacatttc tgaaatttac agaaatatcc gacccctgat
     1321 gtgacctgta aagggcattt gaggatgaaa agagggactt ctgctgacca atcacatctg
     1381 ttaggtaaca gtctagcagt actttccact acagagcaga attggggttg tgatttaagt
     1441 caatgtgtga tgcactaacc ccaggaataa aacattaatc taaatttggg ctattgatga
     1501 taattgactg atgtctgggt ctgtgttttc aactaactgt tatttacttt agccatcatt
     1561 gctaacagac aagtgttgat cggccaccaa taggacattt gctcctgcaa acagataatt
     1621 tgtctatatc aatttcagaa atgcatctcc tttcgtaata tttacaaaaa ttttagccct
     1681 actgtgaaat taatttattt tcagatattt cccacatgct gaaatagcat cttctttttt
     1741 aacaataatt ttaataaata atttcttttg tctttgctat gataattgca cattatacca
     1801 gctattttgc aagatattag tattcagttt aaagtgcaat ttaaaggctt aactagtagg
     1861 ttaaatggag tacatatagg tttcataata ttaagcacta aacattagag gtttatgggg
     1921 cctgttgaaa atatattttt aaataatatt ttacaaacaa actaatagaa ataagaattt
     1981 ctccagaata ataacatagg aaatactgtt taataaatgt gttaaacatc actagggaaa
     2041 tatctgaaaa agaattaaat gtttatctgt gtaaatattt atatactgat gtagcatgac
     2101 ctgtaataat tgctctgtca tgctatatcc atacagtgta gggatgtatg ttgagactac
     2161 tataaatgtt taatcagaca cagattaaaa ttagtaagtg atcttgcggc tcagtaacag
     2221 aggtcacttc taaatccaac agtgtttaaa tacataaacg ttccctttta ttaatatgtt
     2281 tgtgtaagat catgatataa ttatcttaac aagcataaat tgcattcaag ctttaatatc
     2341 ctaatatatt ctaatatcct tatattgaat ctgtaaaaaa aatctgttaa ttaactctac
     2401 agttcaagaa aatgagtttt ttttgacatt ttagttgttt gaaataatat aatgcaaaag
     2461 taataatata taaagaaata agtctgcaga ttatacacgg gtaggttaca aggttttgca
     2521 ccataatata caactccaac ctagccgatt tgtcacttta aactatgcga aaaagtccct
     2581 ttgggaactt aaggttaaaa gttgttggaa gacacatcaa ggtctcataa tgcaattcaa
     2641 aagcataaat aaacattata aaatcttgaa ttattcacag atgattcctt ggatttacaa
     2701 ataattttat gctaagtggt tgtaaacaat gtattttgcc tgaattaaac aaagcaatta
     2761 agttgaatat tattgcattt aattagtttg tttaagttca acacacataa attgtttgca
     2821 acaacttttc agatattgtt ttttcttcag tgtatggtga actgctaatt tatggatatt
     2881 tttaacagtg gatgcattaa aacaattttc agttcttcaa attaagtgtc aaagtttgtg
     2941 tatgatcatg ctataattat ctcaacaaac ataaattgca tttaagctat actatcccac
     3001 atgcactgta caaaatatct gttaattaac agtattttgt gattcacaag tgtttcccct
     3061 ttatttatgg ttgggaattg cattaatgga actttgatct ctgtcgactt ttgatgttga
     3121 aaattcacct acagtttaac aaagtgactt ttattgacat tttaacaagg cataataaat
     3181 tatatgtaga gaaatatatc tgtaaaataa aggaatgcat acgggcagct tggttttgta
     3241 ccatattata cgacaccacc aacccagaca atttgtcact ttaaactatt cgaaatgtta
     3301 gtaagtcact ttttttgaac ttgaaggata aaagtcaaaa acatcaaggt ctcataatgc
     3361 aatttaaaag catgaataat cttttttaca gttgtaaatt ggattatggg acgttggtct
     3421 ctgttctgtt gacttttgat gttgaacttt caacattaca gtttaaagtt tacagtgtct
     3481 taaataacag aaaatgcatg gcagcttttt aaacaagctg tttgtactgc aagatacaac
     3541 accaaccctg acaatatgtc actttaatcc atttaaaatg tttataaaag tcactttgtc
     3601 aaacatttca aggttaaaag ataaagatat gcaattcaaa atcaaaaata aacggggaaa
     3661 aatatcaaat aaaaacataa atcacaaaaa atggaaaact gcaaatttcc ggatatattt
     3721 ttacattgtg gtctgttttg tagaatacca agctttaaat taattaatta aagcatctcc
     3781 agtctaaatc tgactatgca gctcatttat tatattaaat atagccccta aatatgtcca
     3841 agccttgaat gaaagcaacc aaccaacagc atagttagtg agtgtaatgg ggttgcacca
     3901 ttgattcctt ttaaatggaa taaagtatca ccacaactag cagagacaac ttaattacac
     3961 agaacccagc agagtatgaa atcggacatg accgattgat tcttttctga actatcgact
     4021 gctaaaagta gtgccaacat gtggccaaat gccattagaa acttagcttt gataataaca
     4081 actcccttca gctttctggt ttttaagtag ctattaatca aattaaatgt gtaaatgttt
     4141 tagttttatg tctttaaaaa agaaccaata ggtctttagt tctgtagaaa agtgtgtgtg
     4201 tgtttatctc aagttggcac ccatgttggg ctagatttgc agttcagctg aaaaaatcgt
     4261 tctgcaatgg gtgttaacta gcaatcaacc acgcaagact gtctaacatt actttaatcg
     4321 atagcgggtg cacaatagca tgaggcatca agtgtaccca agcgtaaatt cataaagcct
     4381 acagaagttt ttatgtttta tcttatcatt gcagggtttt acttacgcat ggtatacagt
     4441 ggcagtgttg gcaagctgaa tcaattcagt tttcccttca tgttgatagt ggagcactga
     4501 ttattgacgc tctcacatac agaagcagcg cgcttgccaa caaagccctg cgcactcgga
     4561 aaccttcttt tggtgccaac aaagaggaaa gagggggtca caaaagttta ggtgatgtgc
     4621 tgcgtcagat gtgcagggca ccaacgattt ttaaatattt tggtggcctc gacgcgcctc
     4681 tatgacccgc ggggatttaa agcagcggca gactgtccat actgctgtaa ggtaaatggg
     4741 cacattgatt tctcctgcat gactataagt tggcacctag acgacctgct tgttatgtgt
     4801 gtagcccaga cctttaatta taagcgggat tgcatgagtc tagctttttg tagctagtgt
     4861 gtagatgcag gatgggcttg ttacaatgaa gatttacttg atttccccaa cttttgttta
     4921 aggtacgttg atggtttata gttgatttat ttgctttcta ttaaagataa agtcgcatgg
     4981 agttagaact gtaaacacag caattgattt cacttattag caccacatgc aatgtcaatt
     5041 ttttgtgacc cattaataga tgttaattaa gcttgctgta attgtatttc agtacaaaca
     5101 tgcttgtatt taaggatgtt tatgacctaa tttcttggaa catgttaaaa tattttaaca
     5161 aatatatatt tattatcttt aacgttatat tattattata gttattgtta ttaatttaca
     5221 tatttatctg tgactacaca tatatatata tatatatata tttaacacag ctcagattgc
     5281 agaaagaata ttacaattgt ggttctattt aagtatttta aaccatgtta gatacacagc
     5341 taatatgtgg gtggtgcttt attttaaaat ggtaattaat aactaattta tctagaaaaa
     5401 tggtggaaag ttttttttta catgttggtc tgccattaat ctaaattttg ttggagagat
     5461 tgggtttttt tatacttgac tttcagctta taaaatgttt tgaaacatag ggttgagact
     5521 tttgtctgga tttattgttt gcatatttgt gtgaggttac tgttggaaaa cattgatttt
     5581 aaattaaagc tctcaaacat ttaaataaca gttttatact ctggttgatt taatttcaat
     5641 atattttcat atttaaaagc aatttcccat ggtctaggtt catgtttctt tcttcttatt
     5701 ttgtctttac agacacaaaa aagcccgagt aaaggatcca ccggtcacta gttctagagc
     5761 ggccgccacc gcggacaggt ttaaacaatt caagtttgta caaaaaagca ggctggacca
     5821 tggcacccaa gaagaagagg aaggcccaac aatcacccta ttcagcagcg atggcagaac
     5881 agcgtcacca ggagtggtta cgttttgtgg acctgcttaa gaatgcctac caaaacgatc
     5941 tccatttacc gttgttaaac ctgatgctga cgccagatga gcgcaaagcg ttggggactc
     6001 gcgtgcgtat tgtcgaagag ctgttgcgcg gcgaaatgag ccagcgtgag ttaaaaaatg
     6061 aactcggcgc aggcatcgcg acgattacgc gtggatctaa cagcctgaaa gccgcgcccg
     6121 tcgagctgcg ccagtggctg gaagaggtgt tgctgaaaag cgatgggtcg acgctagccg
     6181 gtggcgccaa ttttaatcaa agtgggaata ttgctgatag ctcattgtcc ttcactttca
     6241 ctaacagtag caacggtccg aacctcataa caactcaaac aaattctcaa gcgctttcac
     6301 aaccaattgc ctcctctaac gttcatgata acttcatgaa taatgaaatc acggctagta
     6361 aaattgatga tggtaataat tcaaaaccac tgtcacctgg ttggacggac caaactgcgt
     6421 ataacgcgtt tggaatcact acagggatgt ttaataccac tacaatggat gatgtatata
     6481 actatctatt cgatgatgaa gataccccac caaacccaaa aaaagagggt ctagaattcc
     6541 tcgacggatc gtagatccag acatgataag atacattgat gagtttggac aaaccacacc
     6601 cagctttctt gtacaaagtg ggggatccag acatgataag atacattgat gagtttggac
     6661 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     6721 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     6781 ttatgtttca ggttcagggg gaggtgtggg aggttttttc caactttatt atacatagtt
     6841 gataattcac tggccgtcgt tttacggtac catcgatgat gatccagaca tgataagata
     6901 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     6961 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     7021 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag
     7081 caagtaaaac ctctacaaat gtggtatggc tgattatgat cctctagatc agatccagag
     7141 tcgcggccgc ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcgc
     7201 gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc aacttgatgt
     7261 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
     7321 cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg cccttcaggg
     7381 cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc ttcttctgca
     7441 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcgc
     7501 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aagttcatca
     7561 cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg atgtcggcgg
     7621 ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg tcccaggcga
     7681 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
     7741 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
     7801 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
     7861 tcggtacctg tctgctttgc tgttggtctg ggctcctggg tcactggctt actaatggag
     7921 tctttatgta tgaggactct tatcaattgt tcttctataa aggtctgcag tgtttctgtt
     7981 cgtcccctac atggacaccc agagcctcct aaatacagga gccctgataa ctgcacaagt
     8041 gctcagattc cagcagggtg gaaaatgaga taaagtgtgc agatggggag ggggacgtga
     8101 atgagagatt tgagggatga aaaggatgga tgaacgcatt gaaaatagcc cctttcacaa
     8161 aataatacca gtaaattgtc atataattaa tgaccagacc tttacctgta aatgatgtga
     8221 tgttcatata cagaagaatg ccaacaattt acagatgatt ttacaaattc ttaattttcc
     8281 ttttctaaat ggatttgttc acacattatc tcttcaaagc aattgaattt tctggaaaag
     8341 attggctgtg tgtgtaaaag gtgctactga ggaatttaat gtgacatgga atgaagcaaa
     8401 cagcagccta agacaggatg gcaggaaaaa tgtcacatgt ttaaaataag agtgaaacca
     8461 acgacctgga cttatagaga ctgtgtgtcc ttaggcaaag ctcttgtaca aacagtgctt
     8521 gtgactgtga aattcggggt ttgcctggat tgtgttaaat gtgtgcttgg atgtatcata
     8581 aaaaagaatc ggttctctgt atttgacatt ttctgtaatt tatctaaagt tacaactggc
     8641 tatgcctgat ttttatcact attgaatcaa tcattttaaa gaatgttaag tctgctgatt
     8701 tggagagctc atttacattt attttgaatg tctcttattt aacacaactg atttaagctt
     8761 tagatctgcg aagatacggc cacgggtgct cttgatcctg tggctgattt tggactgtgc
     8821 tgctcgcagc tgctgatgaa tcacatactt cctccatttt cttccactga ttgactgtta
     8881 taatttccct aatttccagg tcaaggtgct gtgcattgtg gtaatagatg tgacatgacg
     8941 tcacttccaa aggaccaatg aacatgtctg accaatttca tataatgtga aaacgatttt
     9001 cataggcaga ataaataaca tttaaattaa actgggcatc agcgcaattc aattggtttg
     9061 gtaatagcaa gggaaaatag aatgaagtga tctccaaaaa ataagtactt tttgactgta
     9121 aataaaattg taaggagtaa aaagtacttt tttttctaaa aaaatgtaat taagtaaaag
     9181 taaaagtatt gatttttaat tgtactcaag taaagtaaaa atccccaaaa ataatactta
     9241 agtacagtaa tcaagtaaaa ttactcaagt actttacacc tctggttctt gaccccctac
     9301 cttcagcaag cccagcagat ccactagttc tagagcggcc gccaccgcgg tggagctcca
     9361 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
     9421 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     9481 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     9541 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     9601 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     9661 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     9721 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     9781 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     9841 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     9901 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     9961 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10021 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10081 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    10141 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    10201 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
    10261 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    10321 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    10381 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    10441 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    10501 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    10561 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    10621 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    10681 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    10741 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    10801 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    10861 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    10921 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    10981 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11041 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    11101 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    11161 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    11221 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    11281 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    11341 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    11401 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    11461 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    11521 aaataggggt tccgcgcaca tttccccgaa aagtg
//
