LOCUS       sequence-168254-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red color variant of bright luminescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Suzuki K, Kimura T, Shinoda H, Bai G, Daniels MJ, Arai Y, Nakano M, 
            Nagai T
  TITLE     Five colour variants of bright luminescent protein for real-time 
            multicolour bioimaging.
  JOURNAL   Nat Commun. 2016 Dec 14;7:13718. doi: 10.1038/ncomms13718.
  PUBMED    27966527
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-12-14"; volume: "7:13718. doi"; pages: " 
            10.1038/ncomms13718"
COMMENT     Sequence Label: ReNL/pcDNA3
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      86..95
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(225..243)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     493..512
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     687..710
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttggta ccgagctcgg atcccgccac catggtgagc aagggcgagg aggtcatcaa
      121 agagttcatg cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat
      181 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
      241 caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc
      301 caaggcgtac gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga
      361 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca
      421 ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt
      481 cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg
      541 cctgtacccc cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga
      601 cggcggccac tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact
      661 gcccggctac tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac
      721 catcgtggaa cagtacgagc gctccgaggg ccgccaccac ctgttcct
//
