LOCUS       sequence-168260-        4200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing KRT26 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_KRT26_p.S2C
FEATURES             Location/Qualifiers
     source          1..4200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1600..1699)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1716..1735)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1717..1735)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1740..1756)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1740..1756)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2187..2978
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3071..3659
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3560..3579
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3813..3830
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3989..4016)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4108..4194)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtgtttt cgactttctg gtggatccag gaggatctgc tcgcgaactg
      241 ggtctggtag gctgtccggt ggaggaacag gcttcgtggc tgggaatgtg tgtgttggat
      301 cgggagcaag aagcagcttt tcttgtactc ttgagggcat ctcttctgga ggaagcttct
      361 gcaatagcgg tggagggttg ggaagtggtg cctgtgctgg ttttcttggc aatgagcaca
      421 gcctcctctc tgggaatgaa aaggtgacca tgcagaatct caacgaccgc ctggcatcct
      481 acctggacca tgtgcatgct ctagaggagg ccaacgcaga cctggagcag aagatcaagg
      541 gctggtacga gaaatgtgag cctggctctt cccgggaaca cgatcatgac tatagcagat
      601 acttctcagt catagaagat cttaaaaggc agattatttc tgcgaccatc tgcaatgcca
      661 gcattgttct acaaaatgac aatgccagac tgaccgctga tgacttcagg ctgaagtatg
      721 aaaatgagct ggctctgcac cacagtgttg aggccgacac cagtggtctt cgcagagtgt
      781 tggatgaact gaccctttgt acaaccgacc tggagataca gtgtgagacc ctcagtgagg
      841 aattgaccta cctcaaaaaa agtcatgagg aggaaatgga agtcttgcaa tatacagctg
      901 gggggaacgt gaacgtggag atgaatgcaa ccccaggagt ggacctaact gtcctgttga
      961 acaacatgag ggctgagtat gaggacttgg ctgagcagaa ccgcaaagat gctgaagcct
     1021 ggttcaacga gaggagtgca acgctgcaac aacagatttc cgatcatgag ggagcagcca
     1081 cagcagccag aaatgagctg accgaattaa aacgcaatct gcaaaccctg gaaatagaac
     1141 ttcagtccct catggctgtg aaacattcct atgaatgctc cttggctgag actgaaggaa
     1201 attactgcaa tcaactccag caaattcagg atcagatagg ggtgatggag gaacaactgc
     1261 aacagattcg aacagaaaca gaaggccaga agctggagta tgaacagctt cttgatgtaa
     1321 aaatattttt agaaaaagaa attgacattt attgcaactt actagatgga gaagaaagaa
     1381 aaagcaaatc cacatgttac aaatcaaaag gatacaggcc tgtgaattct ggaaatcaag
     1441 ccaaagactc aacagaagaa actattgtta aaacagtggt tgaggaactg gatcaaattg
     1501 gcaatctcct ttcactgaga gtccactcag ttgaagaaaa gtcctctaaa ataagcaaca
     1561 ttacagtaga gcaacgagta ccttctaaag caccatgatt gccaactttc ttgtacaaag
     1621 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     1681 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     1741 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     1801 aagataaaaa tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt
     1861 cgctgctacc caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc
     1921 agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca
     1981 actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg
     2041 cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc
     2101 gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca
     2161 gtcgccctaa aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc
     2221 aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac
     2281 atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg
     2341 ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg
     2401 aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg
     2461 tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat
     2521 ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg
     2581 ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg
     2641 aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc
     2701 tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc
     2761 gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg
     2821 caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc
     2881 ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact
     2941 acgtgaaagg cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa
     3001 aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca
     3061 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     3121 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     3181 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     3241 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     3301 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     3361 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     3421 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     3481 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     3541 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3601 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3661 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3721 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3781 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3841 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3901 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
     3961 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
     4021 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
     4081 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
     4141 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
//
