LOCUS       sequence-168263-        4125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing COPS2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_COPS2_p.A317D
FEATURES             Location/Qualifiers
     source          1..4125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1525..1624)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1641..1660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1642..1660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1665..1681)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1665..1681)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2112..2903
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2996..3584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3485..3504
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3738..3755
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3914..3941)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4033..4119)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgtctgac atggaggatg atttcatgtg cgatgatgag gaggactacg
      241 acctggaata ctctgaagat agtaactccg agccaaatgt ggatttggaa aatcagtact
      301 ataattccaa agcattaaaa gaagatgacc caaaagcggc attaagcagt ttccaaaagg
      361 ttttggaact tgaaggtgaa aaaggagaat ggggatttaa agcactgaaa caaatgatta
      421 agattaactt caagttgaca aactttccag aaatgatgaa tagatataag cagctattga
      481 cctatattcg gagtgcagtc acaagaaatt attctgaaaa atccattaat tctattcttg
      541 attatatctc tacttctaaa cagatggatt tactgcagga attctatgaa acaacactgg
      601 aagctttgaa agatgctaag aatgatagac tgtggtttaa gacaaacaca aagcttggaa
      661 aattatattt agaacgagag gaatatggaa agcttcaaaa aattttacgc cagttacatc
      721 agtcgtgcca gactgatgat ggagaagatg atctgaaaaa aggtacacag ttattagaaa
      781 tatatgcttt ggaaattcaa atgtacacag cacagaaaaa taacaaaaaa cttaaagcac
      841 tctatgaaca gtcacttcac atcaagtctg ccatccctca tccactgatt atgggagtta
      901 tcagagaatg tggtggtaaa atgcacttga gggaaggtga atttgaaaag gcacacactg
      961 atttttttga agccttcaag aattatgatg aatctggaag tccaagacga accacttgct
     1021 taaaatattt ggtcttagca aatatgctta tgaaatcggg aataaatcca tttgactcac
     1081 aggaggccaa gccgtacaaa aatgatccag aaattttagc aatgacgaat ttagtaagtg
     1141 actatcagaa taatgacatc actgaatttg aaaagattct aaaaacaaat cacagcaaca
     1201 tcatggatga tcctttcata agagaacaca ttgaagagct tttgcgaaac atcagaacac
     1261 aagtgcttat aaaattaatt aagccttaca caagaataca tattcctttt atttctaagg
     1321 agttaaacat agatgtagct gatgtggaga gcttgctggt gcagtgcata ttggataaca
     1381 ctattcatgg ccgaattgat caagtcaacc aactccttga actggatcat cagaagaggg
     1441 gtggtgcacg atatactgca ctagataaat ggaccaacca actaaattct ctcaaccagg
     1501 ctgtagtcag taaactggct tgattgccaa ctttcttgta caaagttggc attataagaa
     1561 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     1621 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     1681 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     1741 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     1801 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     1861 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     1921 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     1981 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2041 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2101 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     2161 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     2221 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     2281 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2341 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     2401 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     2461 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     2521 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     2581 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     2641 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     2701 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     2761 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     2821 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     2881 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     2941 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3001 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3061 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3121 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3181 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3241 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3301 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3361 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3421 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3481 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3541 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3601 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3661 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3721 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3781 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     3841 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     3901 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     3961 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     4021 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4081 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcct
//
