LOCUS       sequence-168266-        4302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing CYP3A5 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_CYP3A5_p.G177R
FEATURES             Location/Qualifiers
     source          1..4302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1702..1801)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1818..1837)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1819..1837)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1842..1858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1842..1858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2289..3080
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3173..3761
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3662..3681
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3915..3932
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4091..4118)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4210..4296)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggacctc atcccaaatt tggcggtgga aacctggctt ctcctggctg
      241 tcagcctggt gctcctctat ctatatggga cccgtacaca tggacttttt aagagactgg
      301 gaattccagg gcccacacct ctgcctttgt tgggaaatgt tttgtcctat cgtcagggtc
      361 tctggaaatt tgacacagag tgctataaaa agtatggaaa aatgtgggga acgtatgaag
      421 gtcaactccc tgtgctggcc atcacagatc ccgacgtgat cagaacagtg ctagtgaaag
      481 aatgttattc tgtcttcaca aatcgaaggt ctttaggccc agtgggattt atgaaaagtg
      541 ccatctcttt agctgaggat gaagaatgga agagaatacg gtcattgctg tctccaacct
      601 tcaccagcgg aaaactcaag gagatgttcc ccatcattgc ccagtatgga gatgtattgg
      661 tgagaaactt gaggcgggaa gcagagaaag gcaagcctgt cacctggaaa gacatcttta
      721 gggcctacag catggatgtg attactggca catcatttgg agtgaacatc gactctctca
      781 acaatccaca agaccccttt gtggagagca ctaagaagtt cctaaaattt ggtttcttag
      841 atccattatt tctctcaata atactctttc cattccttac cccagttttt gaagcattaa
      901 atgtctctct gtttccaaaa gataccataa attttttaag taaatctgta aacagaatga
      961 agaaaagtcg cctcaacgac aaacaaaagc accgactaga tttccttcag ctgatgattg
     1021 actcccagaa ttcgaaagaa actgagtccc acaaagctct gtctgatctg gagctcgcag
     1081 cccagtcaat aatcttcatt tttgctggct atgaaaccac cagcagtgtt ctttccttca
     1141 ctttatatga actggccact caccctgatg tccagcagaa actgcaaaag gagattgatg
     1201 cagttttgcc caataaggca ccacctacct atgatgccgt ggtacagatg gagtaccttg
     1261 acatggtggt gaatgaaaca ctcagattat tcccagttgc tattagactt gagaggactt
     1321 gcaagaaaga tgttgaaatc aatggggtat tcattcccaa agggtcaatg gtggtgattc
     1381 caacttatgc tcttcaccat gacccaaagt actggacaga gcctgaggag ttccgccctg
     1441 aaaggttcag taagaagaag gacagcatag atccttacat atacacaccc tttggaactg
     1501 gacccagaaa ctgcattggc atgaggtttg ctctcatgaa catgaaactt gctctaatca
     1561 gagtccttca gaacttctcc ttcaaacctt gtaaagaaac acagatcccc ttgaaattag
     1621 acacgcaagg acttcttcaa ccagaaaaac ccattgttct aaaggtggat tcaagagatg
     1681 gaaccctaag tggagaatga ttgccaactt tcttgtacaa agttggcatt ataagaaagc
     1741 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt
     1801 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc
     1861 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
     1921 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg
     1981 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt
     2041 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga
     2101 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt
     2161 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
     2221 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt
     2281 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg
     2341 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag
     2401 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc
     2461 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg
     2521 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc
     2581 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc
     2641 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag
     2701 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc
     2761 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg
     2821 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag
     2881 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg
     2941 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc
     3001 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca
     3061 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt
     3121 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3181 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3241 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3301 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3361 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3421 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3481 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3541 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3601 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3661 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3721 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3781 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3841 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     3901 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     3961 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     4021 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     4081 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     4141 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     4201 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     4261 ggcccagtct tccgactgag cctttcgttt tatttgatgc ct
//
