LOCUS       sequence-168273-        4308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ACVR1B , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ACVR1B_p.D335N
FEATURES             Location/Qualifiers
     source          1..4308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1708..1807)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1824..1843)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1825..1843)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1848..1864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1848..1864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2295..3086
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3179..3767
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3668..3687
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3921..3938
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4097..4124)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4216..4302)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catggcggag tcggccggag cctcctcctt cttccccctt gttgtcctcc
      241 tgctcgccgg cagcggcggg tccgggcccc ggggggtcca ggctctgctg tgtgcgtgca
      301 ccagctgcct ccaggccaac tacacgtgtg agacagatgg ggcctgcatg gtttccattt
      361 tcaatctgga tgggatggag caccatgtgc gcacctgcat ccccaaagtg gagctggtcc
      421 ctgccgggaa gcccttctac tgcctgagct cggaggacct gcgcaacacc cactgctgct
      481 acactgacta ctgcaacagg atcgacttga gggtgcccag tggtcacctc aaggagcctg
      541 agcacccgtc catgtggggc ccggtggagc tggtaggcat catcgccggc ccggtgttcc
      601 tcctgttcct catcatcatc attgttttcc ttgtcattaa ctatcatcag cgtgtctatc
      661 acaaccgcca gagactggac atggaagatc cctcatgtga gatgtgtctc tccaaagaca
      721 agacgctcca ggatcttgtc tacgatctct ccacctcagg gtctggctca gggttacccc
      781 tctttgtcca gcgcacagtg gcccgaacca tcgttttaca agagattatt ggcaagggtc
      841 ggtttgggga agtatggcgg ggccgctgga ggggtggtga tgtggctgtg aaaatattct
      901 cttctcgtga agaacggtct tggttcaggg aagcagagat ataccagacg gtcatgctgc
      961 gccatgaaaa catccttgga tttattgctg ctgacaataa agataatggc acctggacac
     1021 agctgtggct tgtttctgac tatcatgagc acgggtccct gtttgattat ctgaaccggt
     1081 acacagtgac aattgagggg atgattaagc tggccttgtc tgctgctagt gggctggcac
     1141 acctgcacat ggagatcgtg ggcacccaag ggaagcctgg aattgctcat cgaaacttaa
     1201 agtcaaagaa cattctggtg aagaaaaatg gcatgtgtgc catagcagac ctgggcctgg
     1261 ctgtccgtca tgatgcagtc actgacacca ttgacattgc cccgaatcag agggtgggga
     1321 ccaaacgata catggcccct gaagtacttg atgaaaccat taatatgaaa cactttgact
     1381 cctttaaatg tgctgatatt tatgccctcg ggcttgtata ttgggagatt gctcgaagat
     1441 gcaattctgg aggagtccat gaagaatatc agctgccata ttacgactta gtgccctctg
     1501 acccttccat tgaggaaatg cgaaaggttg tatgtgatca gaagctgcgt cccaacatcc
     1561 ccaactggtg gcagagttat gaggcactgc gggtgatggg gaagatgatg cgagagtgtt
     1621 ggtatgccaa cggcgcagcc cgcctgacgg ccctgcgcat caagaagacc ctctcccagc
     1681 tcagcgtgca ggaagacgtg aagatctacc caactttctt gtacaaagtt ggcattataa
     1741 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1801 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     1861 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1921 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     1981 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     2041 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2101 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2161 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2221 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2281 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2341 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2401 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2461 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2521 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2581 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2641 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2701 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2761 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2821 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     2881 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     2941 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3001 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3061 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3121 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3301 gcagagcgca gataccaaat attgttcttc tagtgtagcc gtagttaggc caccacttca
     3361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3541 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     3601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     3661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     3721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3781 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3961 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4021 ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct agccaggaag
     4081 agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag tttgatgcct
     4141 ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca caacgttcaa
     4201 atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa
     4261 acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcct
//
