LOCUS       sequence-168274-        4338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TBL1XR1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TBL1XR1_p.L81V
FEATURES             Location/Qualifiers
     source          1..4338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1738..1837)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1854..1873)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1855..1873)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1878..1894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1878..1894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2325..3116
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3209..3797
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3698..3717
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3951..3968
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4127..4154)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4246..4332)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcac catgagtata agcagtgatg aggtcaactt cttggtatat agatacttgc
      241 aagagtcagg attttctcat tcagcattta cctttggtat agaaagccat atcagtcagt
      301 ccaatataaa tggtgccctc gtcccacccg ctgcattgat ttctatcatc cagaaaggtc
      361 tacagtatgt agaagcagaa gttagtatta atgaggatgg taccttgttt gatggtcgac
      421 caatagagtc tgtgtccctg atagatgccg taatgcctga tgtagtacaa acaagacaac
      481 aagcttatag agataagctt gcacagcaac aggcagcagc tgctgcagct gccgcagctg
      541 cagccagcca acaaggatct gcaaaaaatg gagaaaacac agcaaatggg gaggagaatg
      601 gagcacatac tatagcaaat aatcatactg atatgatgga agtggatggg gatgttgaaa
      661 tccctcctaa taaagctgtt gtgttgcggg gccatgaatc tgaagttttt atctgtgcct
      721 ggaaccctgt tagtgatctc ctagcatcag ggtctggaga ctcaacagca agaatatgga
      781 atcttagtga gaacagcacc agtggctcta cacagttagt acttagacat tgtatacgag
      841 aaggagggca agatgttcca agcaacaagg atgtcacatc tctagattgg aatagtgaag
      901 gtacacttct agcaactggt tcctatgatg ggtttgccag aatatggact aaagatggta
      961 accttgctag caccttaggg cagcataaag gccctatatt tgcattaaaa tggaataaga
     1021 aaggaaattt catcctaagt gctggagtag acaagactac aattatttgg gacgcacata
     1081 ctggtgaagc caagcaacag tttccttttc attcagcacc agcattggat gttgattggc
     1141 agagcaacaa cacctttgct tcttgtagta cagatatgtg cattcatgtc tgtaaattag
     1201 gacaagacag acctattaaa acattccaag gacatacgaa tgaagtaaat gctatcaaat
     1261 gggacccaac tggcaatctc ttggcctcct gttctgacga catgacttta aagatatgga
     1321 gtatgaaaca agacaattgt gtccatgatt tgcaagcaca taataaagaa atttatacta
     1381 tcaaatggag tccaacagga ccagggacta ataatccaaa tgccaacctt atgttagcaa
     1441 gtgcatcctt tgattctact gttaggttat gggatgtaga ccgagggata tgcatccata
     1501 ctttgacaaa acaccaagag cctgtgtaca gtgtagcttt cagtcctgat ggcaggtatc
     1561 tggcaagtgg ttcttttgac aaatgtgtac acatctggaa cacgcagaca ggtgctctag
     1621 ttcacagcta taggggaaca ggtggaatat ttgaagtttg ctggaatgca gcaggagaca
     1681 aagttggagc cagtgcatca gatggttcag tttgtgtatt agaccctcgg aaatgattgc
     1741 caactttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg
     1801 aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata
     1861 gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc
     1921 tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag accagccagg
     1981 acagaaatgc ctcgacttcg ctgctaccca aggttgccgg gtgacgcaca ccgtggaaac
     2041 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt
     2101 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg
     2161 cagtggcggt tttcatggct tgttatgact gtttttttgg ggtacagtct atgcctcggg
     2221 catccaagca gcaagcgcgt tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg
     2281 atgttacgca gcagggcagt cgccctaaaa caaagttaaa cattatgagg gaagcggtga
     2341 tcgccgaagt atcgactcaa ctatcagagg tagttggcgt catcgagcgc catctcgaac
     2401 cgacgttgct ggccgtacat ttgtacggct ccgcagtgga tggcggcctg aagccacaca
     2461 gtgatattga tttgctggtt acggtgaccg taaggcttga tgaaacaacg cggcgagctt
     2521 tgatcaacga ccttttggaa acttcggctt cccctggaga gagcgagatt ctccgcgctg
     2581 tagaagtcac cattgttgtg cacgacgaca tcattccgtg gcgttatcca gctaagcgcg
     2641 aactgcaatt tggagaatgg cagcgcaatg acattcttgc aggtatcttc gagccagcca
     2701 cgatcgacat tgatctggct atcttgctga caaaagcaag agaacatagc gttgccttgg
     2761 taggtccagc ggcggaggaa ctctttgatc cggttcctga acaggatcta tttgaggcgc
     2821 taaatgaaac cttaacgcta tggaactcgc cgcccgactg ggctggcgat gagcgaaatg
     2881 tagtgcttac gttgtcccgc atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg
     2941 atgtcgctgc cgactgggca atggagcgcc tgccggccca gtatcagccc gtcatacttg
     3001 aagctagaca ggcttatctt ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt
     3061 tggaagaatt tgtccactac gtgaaaggcg agatcaccaa ggtagtcggc aaataaccct
     3121 cgagccaccc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     3181 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3241 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3301 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3361 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3421 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3481 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3541 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3601 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3661 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3721 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3781 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3841 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     3901 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     3961 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4021 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4081 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     4141 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     4201 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     4261 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     4321 tcgttttatt tgatgcct
//
