LOCUS       sequence-168275-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  255R. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses 
            GFP and RFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP362
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(47..292)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtctc acttaatttc
       61 tgtactctga agaggagtgg gaaataccaa gaaaaacatc aaactcgaat gattttccca
      121 aacccctacc acaagatatt catcagctgc gagataggct gatcaggagc aagctcgtac
      181 gagaagaaac aaaatgacaa aaaaaatcct atactatata ggttacaaat aaaaaagtat
      241 caaaaatgaa gcctgcatct ctcaggcaaa tggcattctg acatcctctt gattagaatc
      301 tagcaagacc ggtgcggttc cggatccttt ataaagttca tccataccac cagtactgtg
      361 tctcccttcg gctcgttcgt attgctcgac gatggtatag tcttcattat gggaagttat
      421 atccaatttg atgttaacat tgtatgctcc aggcaactgg actggctttt tggccttgta
      481 ggtcgtctta acttcagcat cgtaatgtcc accatcctta agttttagtc tttgcttaat
      541 ttcacctttc aaagctccat cttcgggata catacgttca ctagaagctt cccaacccat
      601 tgtctttttt tgcatcacgg gaccatctga tggaaaattg gtacctctca gcttcacctt
      661 gtagatgaac tcaccgtcct gcagacttga gtcctgagta acagtgacca caccaccgtc
      721 ttcaaagttc attaccctct cccacttaaa accctcaggg aa
//
