LOCUS       sequence-168277-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  255R. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses 
            GFP and RFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP362
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(27..48)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(288..307)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     635..656
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             723..752
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             768..785
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
       61 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      121 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      181 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      241 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      301 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      361 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      421 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
      481 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      541 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagagcg
      601 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
      661 ccgccgggat cactctcggc atggacgagc tgtacaaggc cgcggcggcc gccagctttc
      721 tagaacaaaa actcatctca gaagaggatc tgaatagcgc cgtcgaccat catcatcatc
      781 atcattgacc ggtcttgcta gattctaatc aagaggatgt cagaatgcca tt
//
