LOCUS       sequence-168278-         676 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  255B. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses 
            rHGH and IFNa2b..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP363
FEATURES             Location/Qualifiers
     source          1..676
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(53..299)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgtctcactt
       61 aatcttctgt actctgaaga ggagtgggaa ataccaagaa aaacatcaaa ctcgaatgat
      121 tttcccaaac ccctaccaca agatattcat cagctgcgag ataggctgat caggagcaag
      181 ctcgtacgag aagaaacaaa atgacaaaaa aaatcctata ctatataggt tacaaataaa
      241 aaagtatcaa aaatgaagcc tgcatctctc aggcaaatgg cattctgaca tcctcttgat
      301 tagaatctag caagaccggt tcattctttt gatcgcaatg actcctgcag gtttgtactc
      361 aaagaaaaac ttctcataat ttcggctcta acgacctccc aggcgcaagg tgaatacttt
      421 ttttctttca agtacaaagt aattctttga aagtattttc tgacggccag tatagagtct
      481 tcattcatta ggggggtttc agtaacaccc actccttgaa ttacgcaagc ctccaaatcg
      541 ttaagctgtt gatatagttc agtgtaaaat ttgtccaaaa gggtttcgtc ccaggctgct
      601 gaggaatctt tggttgaaaa caagttaaat atttgctgta tcatctcatg caaaacagga
      661 attgtttcag cttttt
//
