LOCUS       sequence-168283-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 4. Promoter GAP3. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP161
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    239..257
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ccatgaattc tgtgttttga tagttgttca attgattgaa atagggacaa ataaattaaa
       61 tttaaagtct ttgggtcagg agaaaccaaa attgggaaag gtgttcgcct tttatattcg
      121 attctggtgg tttccaataa tctcatgaca tgcgtccgcc cgctattatt gccagcgacg
      181 gccgggactt ttccatccct gggctgctag gtcgggtaca cgacctccgt tttacccgtc
      241 tctatcactg atagggagat ctctatcact gatagggaca acgtaatgct gggaagagca
      301 ttgctgcaag ggggccgtag aagaagctct ccagcagagt aaaatttcct agggacggta
      361 acgggcggtg gaaggagaga gaagggaaga gacgtttctg gttccattac tccacattaa
      421 agttttaccc cggagaattt tacgttgcca gcaggtcgtt cggagttgca acggagccag
      481 atatttcaga gatggctacc tgattggacg aggacaccaa gacatttcta caaaaaagga
      541 tcctgtcaag tgaaaggggc aattttaaga tttcaatctt gatggatttc attgatcgat
      601 aatagtcgca tgtgaggctg aaatgtgccg atgaaagaaa aagaaaaacc ttgcaacaat
      661 ttggtccatt gtcatcactg acctaacgta gttaagggat gctgatattc tttgggaaaa
      721 aaaggaacac catgatccac g
//
