LOCUS       sequence-168284-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: scTEF1, minimal Promoter: mCYC. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP259
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    95..144
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     promoter        265..671
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             695..715
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agatcagatc
       61 tgagctcgtt tggccgtggc cgtgctcgtc ctcgtcggcc ggcttgtcga cgacggcggt
      121 ctccgtcgtc aggatcatcc gggccacaag cttgctgaca gaagcctcaa gaaaaaaaaa
      181 attcttcttc gactatgctg gaggcagaga tgatcgagcc ggtagttaac tatatatagc
      241 taaattggtt ccatcacctc gagtccacac accatagctt caaaatgttt ctactccttt
      301 tttactcttc cagattttct cggactccgc gcatcgccgt accacttcaa aacacccaag
      361 cacagcatac taaatttccc ctctttcttc ctctagggtg tcgttaatta cccgtactaa
      421 aggtttggaa aagaaaaaag agaccgcctc gtttcttttt cttcgtcgaa aaaggcaata
      481 aaaattttta tcacgtttct ttttcttgaa aatttttttt ttgatttttt tctctttcga
      541 tgacctccca ttgatattta agttaataaa cggtcttcaa tttctcaagt ttcagtttca
      601 tttttcttgt tctattacaa ctttttttac ttcttgctca ttagaaagaa agcatagcaa
      661 tctaatctaa ggactagtaa aaatgcgcgg agctcctaag aaaaagcgca aagtccggcc
      721 ggcatctaga cccggggagc gccccttcca gtgtcgcatt tgcatgcgga acttttcgcg
      781 ccaggacagg cttgacaggc atacc
//
