LOCUS       sequence-168286-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: scTEF1, minimal Promoter: mCYC. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP259
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(586..832)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 tgctcaccat gaattcgcgt ttcgatttaa gcttcgaatt gatccggtaa tttagtgtgt
       61 gtatttgtgt ttgcgtgtct atagaagtat agtaatttat gctacaaagg acctaatgta
      121 taaggaaaga atatttagag aaaagaagaa aacaagagtt ttatatacat acagagcaca
      181 tgcatgccat agggtggcgc gcccataggg tatcctcact ccgattgatg aagagaccct
      241 tctggttcca atcctcactc cgattgatgt gacgtcaatg ggtggagtat atcctcactc
      301 cgattgatga gtgctctcga tgacttcgat atcctcactc cgattgatga actccgcccc
      361 attgacgcaa atcctcactc cgattgatgg tggaacagcc caccctgtac atcctcactc
      421 cgattgatgt gcggcctcga gaagcagctg aatcctcact ccgattgatg actggaccgt
      481 caccaattgg agatcctcac tccgattgat ggtggtaaac tcgcaatatc ctcactccga
      541 ttgatgaatt gatctgccgc gatgacttct atatgatatg ctagctctca cttaatcttc
      601 tgtactctga agaggagtgg gaaataccaa gaaaaacatc aaactcgaat gattttccca
      661 aacccctacc acaagatatt catcagctgc gagataggct gatcaggagc aagctcgtac
      721 gagaagaaac aaaatgacaa aaaaaatcct atactatata ggttacaaat aaaaaagtat
      781 caaaaatgaa gcctgcatct ctcaggcaaa tggcattctg acatcctctt gattagaatc
      841 tagcaagacc ggtctaccga tggtacctca a
//
