LOCUS       sequence-168291-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GFP. Figure 3. Strain 5..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP318
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(578..824)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     CDS             complement(866..892)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ccatgaattc gcgtttcgat ttaagcttcg aattgatccg gtaatttagt gtgtgtattt
       61 gtgtttgcgt gtctatagaa gtatagtaat ttatgctaca aaggacctaa tgtataagga
      121 aagaatattt agagaaaaga agaaaacaag agttttatat acatacagag cacatgcatg
      181 ccatagggtg gcgcgccata gggtatgaca ctctcctggt tccgattctc accccaattg
      241 aagtcacatc gattggagga gtgtaccctt attcagactg gtgagtggac tcgataactt
      301 ctatgtcctc ggatctgttg ataaattcgg ccccattcaa cagattctga caccgataga
      361 tgatcctcac tccgattgat gggggaacgg cccatccggt gcacccttac tctgactggt
      421 gagcagcttc cagcaacaat tgaatacgaa ctccgattga actttgaaca gtaaccaatt
      481 gtaaatcctc acttctatta atagtagtaa attcacaata tcctcactcc gattgatgaa
      541 ttgatctgcc gcgatgactt ctatatgata tgctagctct cacttaatct tctgtactct
      601 gaagaggagt gggaaatacc aagaaaaaca tcaaactcga atgattttcc caaaccccta
      661 ccacaagata ttcatcagct gcgagatagg ctgatcagga gcaagctcgt acgagaagaa
      721 acaaaatgac aaaaaaaatc ctatactata taggttacaa ataaaaaagt atcaaaaatg
      781 aagcctgcat ctctcaggca aatggcattc tgacatcctc ttgattagaa tctagcaaga
      841 ccggtctacc gatggtacct caagaagcgt agtccggaac gtcgtacggg tagttaatta
      901 acatatcgag atcgaaatcg tccagagcat cggagccgag catgtccaga tca
//
