LOCUS       sequence-168294-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplemental Figure 4. Promoter GAP7. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP153
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    16..34
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(70..546)
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris 
                     glyceraldehyde-3-phosphate dehydrogenase"
ORIGIN
        1 gctcaccatg aattctctct atcactgata gggagatctc tatcactgat agggaaattc
       61 tgtgttttga tagttgttca attgattgaa atagggacaa ataaattaaa tttaaagtct
      121 ttgggtcagg ggaaaccaaa attgggaaag gtgttcgcct tttatattcg attctggtgg
      181 tttccaataa tctcatgaca tgcgtccgcc cgctattatt gccagcgacg gccgggactt
      241 ttccatccct gggctgctag gtcgggtaca cgacctccgt tttacccgca acgtaatgct
      301 gggaagagca ttgctgcaag ggggccgtag aagaagctct ccagcagagt aaaatttcct
      361 agggacggta acgggcggtg gaaggagaga gaagggaaga gacgtttctg gttccattac
      421 tccacattaa agttttaccc cggagaattt tacgttgcca gcaggtcgtt cggagttgca
      481 acggagccag atatttcaga gatggctacc tgattggacg aggacaccaa gacatttcta
      541 caaaaaagga tcctgtcaag tgaaaggggc aattttaaga tttcaatctt gatggatttc
      601 attgatcgat aatagtcgca tgtgaggctg aaatgtgccg atgaaagaaa aagaaaaacc
      661 ttgcaacaat ttggtccatt gtcatcactg acctaacgta gttaagggat gctgatattc
      721 tttgggaaaa aaaggaacac catgatccac ggtggaagac tataattcag gaacggatgt
      781 caaatgtccg tttatgggac catttcgatg gtaatttgat cggaactact gga
//
