LOCUS       sequence-168297-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: GAP, minimal Promoter: GCW14. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP275
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    110..159
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
       61 tcatgagatc agatctgagc tcgtttggcc gtggccgtgc tcgtcctcgt cggccggctt
      121 gtcgacgacg gcggtctccg tcgtcaggat catccgggcc acaagcttgc tgacagaagc
      181 ctcaagaaaa aaaaaattct tcttcgacta tgctggaggc agagatgatc gagccggtag
      241 ttaactatat atagctaaat tggttccatc acctcgagtc aattcttgat ttagtataca
      301 cataaccaaa tttggatcaa gtttgaagta aaactttaac ttcagctcct tacatttgca
      361 ctaagatctc tgctactctg gtcccaagtg aaccaccttt tggaccctat tgaccggacc
      421 ttaacttgcc aaacctaaac gcttaatgcc tcagacgttt taatgcctct caacacctcc
      481 aaggttgctt tcttgagcat gcctactagg gactttaacg aactgtgggg ttgcagacag
      541 tttcaggcgt gtcccgacca atatggccta ctagactctc tgaaaaatca cagttttcca
      601 gtagttccga tcaaattacc atcgaaatgg tcccataaac ggacatttga catccgttcc
      661 tgaattatag tcttccaccg tggatcatgg tgttcctttt tttcccaaag aatatcagca
      721 tcccttaact acgttaggtc agtgatgaca atggaccaaa ttgttgcaag gtttttcttt
      781 ttctttcatc ggcacatttc agcctcacat gcgactatta tcgatcaatg aaatccatca
      841 agattgaaat cttaaaattg ccc
//
