LOCUS       sequence-168301-        4605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing NFE2L2 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_NFE2L2_WT
FEATURES             Location/Qualifiers
     source          1..4605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..65
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     57..74
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     58..74
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    90..189
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2005..2104)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2121..2140)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2122..2140)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2145..2161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2145..2161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2592..3383
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3476..4064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3965..3984
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4218..4235
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4394..4421)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4513..4599)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
       61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      181 aagttggcat gatggacttg gagctgccgc cgccgggact cccgtcccag caggacatgg
      241 atttgattga catactttgg aggcaagata tagatcttgg agtaagtcga gaagtatttg
      301 acttcagtca gcgacggaaa gagtatgagc tggaaaaaca gaaaaaactt gaaaaggaaa
      361 gacaagaaca actccaaaag gagcaagaga aagccttttt cgctcagtta caactagatg
      421 aagagacagg tgaatttctc ccaattcagc cagcccagca catccagtca gaaaccagtg
      481 gatctgccaa ctactcccag gttgcccaca ttcccaaatc agatgctttg tactttgatg
      541 actgcatgca gcttttggcg cagacattcc cgtttgtaga tgacaatgag gtttcttcgg
      601 ctacgtttca gtcacttgtt cctgatattc ccggtcacat cgagagccca gtcttcattg
      661 ctactaatca ggctcagtca cctgaaactt ctgttgctca ggtagcccct gttgatttag
      721 acggtatgca acaggacatt gagcaagttt gggaggagct attatccatt cctgagttac
      781 agtgtcttaa tattgaaaat gacaagctgg ttgagactac catggttcca agtccagaag
      841 ccaaactgac agaagttgac aattatcatt tttactcatc tataccctca atggaaaaag
      901 aagtaggtaa ctgtagtcca cattttctta atgcttttga ggattccttc agcagcatcc
      961 tctccacaga agaccccaac cagttgacag tgaactcatt aaattcagat gccacagtca
     1021 acacagattt tggtgatgaa ttttattctg ctttcatagc tgagcccagt atcagcaaca
     1081 gcatgccctc acctgctact ttaagccatt cactctctga acttctaaat gggcccattg
     1141 atgtttctga tctatcactt tgcaaagctt tcaaccaaaa ccaccctgaa agcacagcag
     1201 aattcaatga ttctgactcc ggcatttcac taaacacaag tcccagtgtg gcatcaccag
     1261 aacactcagt ggaatcttcc agctatggag acacactact tggcctcagt gattctgaag
     1321 tggaagagct agatagtgcc cctggaagtg tcaaacagaa tggtcctaaa acaccagtac
     1381 attcttctgg ggatatggta caacccttgt caccatctca ggggcagagc actcacgtgc
     1441 atgatgccca atgtgagaac acaccagaga aagaattgcc tgtaagtcct ggtcatcgga
     1501 aaaccccatt cacaaaagac aaacattcaa gccgcttgga ggctcatctc acaagagatg
     1561 aacttagggc aaaagctctc catatcccat tccctgtaga aaaaatcatt aacctccctg
     1621 ttgttgactt caacgaaatg atgtccaaag agcagttcaa tgaagctcaa cttgcattaa
     1681 ttcgggatat acgtaggagg ggtaagaata aagtggctgc tcagaattgc agaaaaagaa
     1741 aactggaaaa tatagtagaa ctagagcaag atttagatca tttgaaagat gaaaaagaaa
     1801 aattgctcaa agaaaaagga gaaaatgaca aaagccttca cctactgaaa aaacaactca
     1861 gcaccttata tctcgaagtt ttcagcatgc tacgtgatga agatggaaaa ccttattctc
     1921 ctagtgaata ctccctgcag caaacaagag atggcaatgt tttccttgtt cccaaaagta
     1981 agaagccaga tgttaagaaa aactacccaa ctttcttgta caaagttggc attataagaa
     2041 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     2101 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     2161 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     2221 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg
     2281 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct
     2341 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
     2401 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
     2461 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc
     2521 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa
     2581 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     2641 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     2701 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     2761 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     2821 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     2881 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     2941 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     3001 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     3061 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     3121 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     3181 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     3241 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     3301 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     3361 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga
     3421 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     3481 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     3541 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3601 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     3661 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3721 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3781 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3841 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3901 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3961 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     4021 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     4081 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     4141 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     4201 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     4261 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     4321 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     4381 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     4441 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     4501 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     4561 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcct
//
