LOCUS       sequence-168302-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP
            and RFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP310
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..23
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctcctaagaa aaagcgcaaa gtccggccgg catctagacc cggggagcgc cccttccagt
       61 gtcgcatttg catgcggaac ttttcgcgcc aggacaggct tgacaggcat acccgtactc
      121 ataccggtga aaaaccgttt cagtgtcgga tctgtatgcg aaatttctcc cagaaggagc
      181 acttggcggg gcatctacgt acgcacaccg gcgagaagcc attccaatgc cgaatatgca
      241 tgcgcaactt cagtcgccgc gacaacctga accggcacct aaaaacccac ctgaggaaca
      301 tatgcggcgg aggcacacct gcagctgcgt cgactctaga ggatccatct gctggagaca
      361 tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag aagaacagcc
      421 tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct gagcccccca
      481 tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg atgggcttac
      541 tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag agggtgccag
      601 gctttgtgga tttgaccctc catgatcagg tccaccttct agaatgtgcc tggctagaga
      661 tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta ctgtttgctc
      721 ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg gagatcttcg
      781 acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcag
//
