LOCUS       sequence-168303-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP
            and RFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP310
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     287..308
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             375..404
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             420..437
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      461..707
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
       61 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
      121 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
      181 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
      241 gcacccagag cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
      301 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag gccgcggcgg
      361 ccgccagctt tctagaacaa aaactcatct cagaagagga tctgaatagc gccgtcgacc
      421 atcatcatca tcatcattga ccggtcttgc tagattctaa tcaagaggat gtcagaatgc
      481 catttgcctg agagatgcag gcttcatttt tgatactttt ttatttgtaa cctatatagt
      541 ataggatttt ttttgtcatt ttgtttcttc tcgtacgagc ttgctcctga tcagcctatc
      601 tcgcagctga tgaatatctt gtggtagggg tttgggaaaa tcattcgagt ttgatgtttt
      661 tcttggtatt tcccactcct cttcagagta cagaagatta agtgagacct tcgtttgtgc
      721 ggatccccca cacaccatag cttcaaaatg tttctactcc ttttttactc ttccagattt
      781 tctcggactc cgcgcatcgc c
//
