LOCUS       sequence-168304-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: GAP6, minimal Promoter: mCYC. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP258
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(578..824)
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 atgaattcgc gtttcgattt aagcttcgaa ttgatccggt aatttagtgt gtgtatttgt
       61 gtttgcgtgt ctatagaagt atagtaattt atgctacaaa ggacctaatg tataaggaaa
      121 gaatatttag agaaaagaag aaaacaagag ttttatatac atacagagca catgcatgcc
      181 atagggtggc gcgcccatag ggtatcctca ctccgattga tgaagagacc cttctggttc
      241 caatcctcac tccgattgat gtgacgtcaa tgggtggagt atatcctcac tccgattgat
      301 gagtgctctc gatgacttcg atatcctcac tccgattgat gaactccgcc ccattgacgc
      361 aaatcctcac tccgattgat ggtggaacag cccaccctgt acatcctcac tccgattgat
      421 gtgcggcctc gagaagcagc tgaatcctca ctccgattga tgactggacc gtcaccaatt
      481 ggagatcctc actccgattg atggtggtaa actcgcaata tcctcactcc gattgatgaa
      541 ttgatctgcc gcgatgactt ctatatgata tgctagctct cacttaatct tctgtactct
      601 gaagaggagt gggaaatacc aagaaaaaca tcaaactcga atgattttcc caaaccccta
      661 ccacaagata ttcatcagct gcgagatagg ctgatcagga gcaagctcgt acgagaagaa
      721 acaaaatgac aaaaaaaatc ctatactata taggttacaa ataaaaaagt atcaaaaatg
      781 aagcctgcat ctctcaggca aatggcattc tgacatcctc ttgattagaa tctagcaaga
      841 ccggtctac
//
