LOCUS       sequence-168305-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Promoter: GAP6, minimal Promoter: mCYC. Figure 4. Expresses GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Perez-Pinera P, Han N, Cleto S, Cao J, Purcell O, Shah KA, Lee K, 
            Ram R, Lu TK
  TITLE     Synthetic biology and microbioreactor platforms for programmable 
            production of biologics at the point-of-care.
  JOURNAL   Nat Commun. 2016 Jul 29;7:12211. doi: 10.1038/ncomms12211.
  PUBMED    27470089
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-29"; volume: "7:12211. doi"; pages: " 
            10.1038/ncomms12211"
COMMENT     Sequence Label: PP258
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    111..160
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
ORIGIN
        1 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
       61 gtcatgagat cagatctgag ctcgtttggc cgtggccgtg ctcgtcctcg tcggccggct
      121 tgtcgacgac ggcggtctcc gtcgtcagga tcatccgggc cacaagcttg ctgacagaag
      181 cctcaagaaa aaaaaaattc ttcttcgact atgctggagg cagagatgat cgagccggta
      241 gttaactata tatagctaaa ttggttccat cacctcgagt ctattcttga tttagtatac
      301 acataaccaa atttggatca agtttgaagt aaaactttaa cttcagctcc ttacatttgc
      361 actaagatct ctgctactct ggtcccaagt gaaccacctt ttggacccta ttgaccggac
      421 cttaacttgc caaacctaaa cgcttaatgc ctcagacgtt ttaatgcctc tcaacacctc
      481 caaggttgct ttcttgagca tgcctactag ggactttaac gaactgtggg gttgcagaca
      541 gtttcaggcg tgtcccgacc aatatggcct actagactct ctgaaaaatc acagttttcc
      601 agtagttccg atcaaattac catcgaaatg gtcccataaa cggacatttg acatccgttc
      661 ctgaattata gtcttccacc gtggatcatg gtgttccttt ttttcccaaa gaatatcagc
      721 atcccttaac tacgttaggt cagtgatgac aatggaccaa attgttgcaa ggtttttctt
      781 tttctttcat cggcacattt cagcctcaca tgcgactatt atcgatcaat gaaatccatc
      841 aagattgaaa tcttaaaatt gcccctttca cttgacagga tcctt
//
