LOCUS       sequence-168320-        1287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pEZT-BM vector with beta2 gene after CMV promoter, published 
            crystallization construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Morales-Perez CL, Noviello CM, Hibbs RE
  TITLE     X-ray structure of the human alpha4beta2 nicotinic receptor.
  JOURNAL   Nature. 2016 Oct 3;538(7625):411-415. doi: 10.1038/nature19785.
  PUBMED    27698419
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-10-3- 3"; volume: "538"; issue: "7625"; pages:
            "411-415"
COMMENT     Sequence Label: Human b2 nicotinic subunit crystallization con...
FEATURES             Location/Qualifiers
     source          1..1287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1261..1284
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 atggcccggc gctgcggccc cgtggcgctg ctccttggct tcggcctcct ccggctgtgc
       61 tcaggggtgt ggggtacgga tacagaggag cggctggtgg agcatctcct ggatccttcc
      121 cgctacaaca agcttatccg cccagccacc aatggctctg agctggtgac agtacagctt
      181 atggtgtcac tggcccagct catcagtgtg catgagcggg agcagatcat gaccaccaat
      241 gtctggctga cccaggagtg ggaagattat cgcctcacct ggaagcctga agagtttgac
      301 aacatgaaga aagttcggct cccttccaaa cacatctggc tcccagatgt ggtcctgtac
      361 aacaatgctg acggcatgta cgaggtgtcc ttctattcca atgccgtggt ctcctatgat
      421 ggcagcatct tctggctgcc gcctgccatc tacaagagcg catgcaagat tgaagtaaag
      481 cacttcccat ttgaccagca gaactgcacc atgaagttcc gttcgtggac ctacgaccgc
      541 acagagatcg acttggtgct gaagagtgag gtggccagcc tggacgactt cacacctagt
      601 ggtgagtggg acatcgtggc gctgccgggc cggcgcaacg agaaccccga cgactctacg
      661 tacgtggaca tcacgtatga cttcatcatt cgccgcaagc cgctcttcta caccatcaac
      721 ctcatcatcc cctgtgtgct catcacctcg ctagccatcc ttgtcttcta cctgccatcc
      781 gactgtggcg agaagatgac gttgtgcatc tcagtgctgc tggcgctcac ggtcttcctg
      841 ctgctcatct ccaagatcgt gcctcccacc tccctcgacg tgccgctcgt cggcaagtac
      901 ctcatgttca ccatggtgct tgtcaccttc tccatcgtca ccagcgtgtg cgtgctcaac
      961 gtgcaccacc gctcgcccac cacgcacacc atggcgccct gggtgaaggt cgtcttcctg
     1021 gagaagctgc ccgcgctgct cttcatgcag cagccacgcc atcatgacga tgaccaggaa
     1081 agaagcgtga gtgaggactg gaagtacgtc gccatggtga tcgaccgcct cttcctctgg
     1141 atctttgtct ttgtctgtgt ctttggcacc atcggcatgt tcctgcagcc tctcttccag
     1201 aactacacca ccaccacctt cctccactca gaccactcag cccccagctc caagtccgcc
     1261 tggtcccacc cccagttcga gaagtga
//
