LOCUS       sequence-168324-        4503 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing ARSI , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_ARSI_p.V373M
FEATURES             Location/Qualifiers
     source          1..4503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2382..2481)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2498..2517)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2499..2517)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2522..2538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2522..2538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2969..3760
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3853..4441
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4342..4361
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgcacaccc tcactggctt ctccctggtc agcctgctca gcttcggcta
      721 cctgtcctgg gactgggcca agccgagctt cgtggccgac gggcccgggg aggctggcga
      781 gcagccctcg gccgctccgc cccagcctcc ccacatcatc ttcatcctca cggacgacca
      841 aggctaccac gacgtgggct accatggttc agatatcgag acccctacgc tggacaggct
      901 ggcggccaag ggggtcaagt tggagaatta ttacatccag cccatctgca cgccttcgcg
      961 gagccagctc ctcactggca ggtaccagat ccacacagga ctccagcatt ccatcatccg
     1021 cccacagcag cccaactgcc tgcccctgga ccaggtgaca ctgccacaga agctgcagga
     1081 ggcaggttat tccacccata tggtgggcaa gtggcacctg ggcttctacc ggaaggagtg
     1141 tctgcccacc cgtcggggct tcgacacctt cctgggctcg ctcacgggca atgtggacta
     1201 ttacacctat gacaactgtg atggcccagg cgtgtgcggc ttcgacctgc acgagggtga
     1261 gaatgtggcc tgggggctca gcggccagta ctccactatg ctttacgccc agcgcgccag
     1321 ccatatcctg gccagccaca gccctcagcg tcccctcttc ctctatgtgg ccttccaggc
     1381 agtacacaca cccctgcagt cccctcgtga gtacctgtac cgctaccgca ccatgggcaa
     1441 tgtggcccgg cggaagtacg cggccatggt gacctgcatg gatgaggctg tgcgcaacat
     1501 cacctgggcc ctcaagcgct acggtttcta caacaacagt gtcatcatct tctccagtga
     1561 caatggtggc cagactttct cggggggcag caactggccg ctccgaggac gcaagggcac
     1621 ttattgggaa ggtggcgtgc ggggcctagg ctttgtccac agtcccctgc tcaagcgaaa
     1681 gcaacggaca agccgggcac tgatgcacat cactgactgg tacccgaccc tggtgggtct
     1741 ggcaggtggt accacctcag cagccgatgg gctagatggc tacgacatgt ggccggccat
     1801 cagcgagggc cgggcctcac cacgcacgga gatcctgcac aacattgacc cactctacaa
     1861 ccatgcccag catggctccc tggagggcgg ctttggcatc tggaacaccg ccgtgcaggc
     1921 tgccatccgc gtgggtgagt ggaagctgct gacaggagac cccggctatg gcgattggat
     1981 cccaccgcag acactggcca ccttcccggg tagctggtgg aacctggaac gaatggccag
     2041 tgtccgccag gccgtgtggc tcttcaacat cagtgctgac ccttatgaac gggaggacct
     2101 ggctggccag cggcctgatg tggtccgcac cctgctggct cgcctggccg aatataaccg
     2161 cacagccatc ccggtacgct acccagctga gaacccccgg gctcatcctg actttaatgg
     2221 gggtgcttgg gggccctggg ccagtgatga ggaagaggag gaagaggaag ggagggctcg
     2281 aagcttctcc cggggtcgtc gcaagaaaaa atgcaagatt tgcaagcttc gatccttttt
     2341 ccgtaaactc aacaccaggc taatgtccca acggatctga ttgccaactt tcttgtacaa
     2401 agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
     2461 tcaaaataaa atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat
     2521 ggtcatagct gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc
     2581 acaagataaa aatatatcat catgcctcct ctagaccagc caggacagaa atgcctcgac
     2641 ttcgctgcta cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac
     2701 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
     2761 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     2821 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     2881 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     2941 cagtcgccct aaaacaaagt taaacattat gagggaagcg gtgatcgccg aagtatcgac
     3001 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     3061 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     3121 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     3181 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     3241 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     3301 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     3361 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     3421 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     3481 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     3541 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     3601 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     3661 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     3721 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa ccctcgagcc acccatgacc
     3781 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3841 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3901 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3961 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     4021 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4081 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4141 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4201 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4261 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4321 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4381 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4441 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4501 gtt
//
