LOCUS       sequence-168325-        4338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway Donor vector containing TBL1XR1 , part of the Target 
            Accelerator Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-16-0160"; journalName: "Cancer Discov"; date: 
            "2016-07"; volume: "6"; issue: "7"; pages: "714-26"
COMMENT     Sequence Label: pDONR223_TBL1XR1_p.T349M
FEATURES             Location/Qualifiers
     source          1..4338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2217..2316)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2333..2352)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2334..2352)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2357..2373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2357..2373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2804..3595
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      3688..4276
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4177..4196
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agttggcacc atgagtataa gcagtgatga ggtcaacttc ttggtatata gatacttgca
      721 agagtcagga ttttctcatt cagcatttac ctttggtata gaaagccata tcagtcagtc
      781 caatataaat ggtgccctcg tcccacccgc tgcattgatt tctatcatcc agaaaggtct
      841 acagtatgta gaagcagaag ttagtattaa tgaggatggt accttgtttg atggtcgacc
      901 aatagagtct ctgtccctga tagatgccgt aatgcctgat gtagtacaaa caagacaaca
      961 agcttataga gataagcttg cacagcaaca ggcagcagct gctgcagctg ccgcagctgc
     1021 agccagccaa caaggatctg caaaaaatgg agaaaacaca gcaaatgggg aggagaatgg
     1081 agcacatact atagcaaata atcatactga tatgatggaa gtggatgggg atgttgaaat
     1141 ccctcctaat aaagctgttg tgttgcgggg ccatgaatct gaagttttta tctgtgcctg
     1201 gaaccctgtt agtgatctcc tagcatcagg gtctggagac tcaacagcaa gaatatggaa
     1261 tcttagtgag aacagcacca gtggctctac acagttagta cttagacatt gtatacgaga
     1321 aggagggcaa gatgttccaa gcaacaagga tgtcacatct ctagattgga atagtgaagg
     1381 tacacttcta gcaactggtt cctatgatgg gtttgccaga atatggacta aagatggtaa
     1441 ccttgctagc accttagggc agcataaagg ccctatattt gcattaaaat ggaataagaa
     1501 aggaaatttc atcctaagtg ctggagtaga caagactaca attatttggg acgcacatac
     1561 tggtgaagcc aagcaacagt ttccttttca ttcagcacca gcattggatg ttgattggca
     1621 gagcaacaac acctttgctt cttgtagtac agatatgtgc attcatgtct gtaaattagg
     1681 acaagacaga cctattaaaa cattccaagg acatatgaat gaagtaaatg ctatcaaatg
     1741 ggacccaact ggcaatctct tggcctcctg ttctgacgac atgactttaa agatatggag
     1801 tatgaaacaa gacaattgtg tccatgattt gcaagcacat aataaagaaa tttatactat
     1861 caaatggagt ccaacaggac cagggactaa taatccaaat gccaacctta tgttagcaag
     1921 tgcatccttt gattctactg ttaggttatg ggatgtagac cgagggatat gcatccatac
     1981 tttgacaaaa caccaagagc ctgtgtacag tgtagctttc agtcctgatg gcaggtatct
     2041 ggcaagtggt tcttttgaca aatgtgtaca catctggaac acgcagacag gtgctctagt
     2101 tcacagctat aggggaacag gtggaatatt tgaagtttgc tggaatgcag caggagacaa
     2161 agttggagcc agtgcatcag atggttcagt ttgtgtatta gaccctcgga aatgattgcc
     2221 aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2281 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2341 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2401 ctgatgttac attgcacaag ataaaaatat atcatcatgc ctcctctaga ccagccagga
     2461 cagaaatgcc tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg
     2521 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     2581 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     2641 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     2701 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     2761 tgttacgcag cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat
     2821 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     2881 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     2941 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     3001 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     3061 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     3121 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     3181 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     3241 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     3301 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     3361 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     3421 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     3481 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     3541 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataaccctc
     3601 gagccaccca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3661 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3721 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3781 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3841 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3901 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3961 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4021 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4081 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4141 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4201 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4261 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4321 gccttttgct cacatgtt
//
